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Glutamine's protection against sepsis and lung injury is dependent on heat shock protein 70 expression.

Publication ,  Journal Article
Singleton, KD; Wischmeyer, PE
Published in: Am J Physiol Regul Integr Comp Physiol
May 2007

Glutamine (GLN) has been shown to protect against inflammatory injury and illness in experimental and clinical settings. The mechanism of this protection is unknown; however, laboratory and clinical trial data have indicated a relationship between GLN-mediated protection and enhanced heat shock protein 70 (HSP70) expression. The aim of this study was to examine the hypothesis that GLN's beneficial effect on survival, tissue injury, and inflammatory response after inflammatory injury is dependent on HSP70 expression. Mice with a specific deletion of the HSP70 gene underwent cecal ligation and puncture (CLP)-induced sepsis and were treated with GLN (0.75 g/kg) or a saline placebo 1 h post-CLP. Lung tissue NF-kappaB activation, inflammatory cytokine response, and lung injury were assessed post-CLP. Survival was assessed for 5 days post-CLP. Our results indicate that GLN administration improved survival in Hsp70(+/+) mice vs. Hsp70(+/+) mice not receiving GLN; however, GLN exerted no survival benefit in Hsp70(-/-) mice. This was accompanied by a significant decrease in lung injury, attenuation of NF-kappaB activation, and proinflammatory cytokine expression in GLN-treated Hsp70(+/+) mice vs. Hsp70(+/+) mice not receiving GLN. In the Hsp70(-/-) mice, GLN's attenuation of lung injury, NF-kappaB activation, and proinflammatory cytokine expression was lost. These results confirm our hypothesis that HSP70 expression is required for GLN's effects on survival, tissue injury, and the inflammatory response after global inflammatory injury.

Duke Scholars

Published In

Am J Physiol Regul Integr Comp Physiol

DOI

ISSN

0363-6119

Publication Date

May 2007

Volume

292

Issue

5

Start / End Page

R1839 / R1845

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Sepsis
  • Physiology
  • NF-kappa B
  • Mice, Knockout
  • Mice
  • Male
  • Lung Diseases
  • Lung
  • Interleukin-6
 

Citation

APA
Chicago
ICMJE
MLA
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Singleton, K. D., & Wischmeyer, P. E. (2007). Glutamine's protection against sepsis and lung injury is dependent on heat shock protein 70 expression. Am J Physiol Regul Integr Comp Physiol, 292(5), R1839–R1845. https://doi.org/10.1152/ajpregu.00755.2006
Singleton, Kristen D., and Paul E. Wischmeyer. “Glutamine's protection against sepsis and lung injury is dependent on heat shock protein 70 expression.Am J Physiol Regul Integr Comp Physiol 292, no. 5 (May 2007): R1839–45. https://doi.org/10.1152/ajpregu.00755.2006.
Singleton KD, Wischmeyer PE. Glutamine's protection against sepsis and lung injury is dependent on heat shock protein 70 expression. Am J Physiol Regul Integr Comp Physiol. 2007 May;292(5):R1839–45.
Singleton, Kristen D., and Paul E. Wischmeyer. “Glutamine's protection against sepsis and lung injury is dependent on heat shock protein 70 expression.Am J Physiol Regul Integr Comp Physiol, vol. 292, no. 5, May 2007, pp. R1839–45. Pubmed, doi:10.1152/ajpregu.00755.2006.
Singleton KD, Wischmeyer PE. Glutamine's protection against sepsis and lung injury is dependent on heat shock protein 70 expression. Am J Physiol Regul Integr Comp Physiol. 2007 May;292(5):R1839–R1845.

Published In

Am J Physiol Regul Integr Comp Physiol

DOI

ISSN

0363-6119

Publication Date

May 2007

Volume

292

Issue

5

Start / End Page

R1839 / R1845

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Sepsis
  • Physiology
  • NF-kappa B
  • Mice, Knockout
  • Mice
  • Male
  • Lung Diseases
  • Lung
  • Interleukin-6