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Glutamine attenuates endotoxin-induced lung metabolic dysfunction: potential role of enhanced heat shock protein 70.

Publication ,  Journal Article
Singleton, KD; Serkova, N; Banerjee, A; Meng, X; Gamboni-Robertson, F; Wischmeyer, PE
Published in: Nutrition
February 2005

OBJECTIVE: Septic shock leads to derangement of cellular metabolism. Enhanced heat shock protein 70 (HSP-70) can preserve cellular metabolism after other forms of cellular stress. Glutamine (GLN) can enhance lung HSP-70 expression after lethal endotoxemia. However, it is unknown whether GLN can enhance HSP-70 expression and attenuate lung metabolic dysfunction after sublethal endotoxemia. Our aim was to determine whether GLN could upregulate HSP-70 and attenuate metabolic dysfunction in lung tissue after sublethal endotoxemia. METHODS: Sprague-Dawley rats were assigned to one of five groups. The first two groups were treated with Escherichia coli lipopolysaccharide (LPS; 1 mg/kg intravenously). GLN (0.75 g/kg intravenously) or balanced salt solution as a control was administered 5 min after LPS administration. The next two groups of rats were treated with quercetin (HSP-70 inhibitor; 400 mg/kg intraperitoneally) 6 h before LPS administration. The final group received no treatment. Lung tissue was harvested 24-h after LPS and analyzed with immunofluorescence and western blot for HSP-70. Tissue metabolites were quantified by 1H and 31P nuclear magnetic resonance spectroscopy. RESULTS: GLN compared with balanced salt solution (BSS) administration in LPS-treated animals led to significant increases in lung HSP-70. Increased HSP-70 expression was observed in lung epithelial cells and macrophages. GLN significantly improved the ratio of adenosine triphosphate to adenosine diphosphate in the lung after LPS. Quercetin inhibited a GLN-mediated increase in lung HSP-70 and blocked a beneficial effect of GLN on the ratio of adenosine triphosphate to adenosine diphosphate after LPS. CONCLUSIONS: A single dose of GLN can enhance HSP-70 in pulmonary epithelial cells and macrophages after sublethal endotoxemia. Further, GLN can attenuate endotoxin-induced lung metabolic dysfunction. GLN's beneficial effect on lung tissue after metabolic dysfunction caused by sublethal endotoxemia may be mediated in part by enhanced HSP-70.

Duke Scholars

Published In

Nutrition

DOI

ISSN

0899-9007

Publication Date

February 2005

Volume

21

Issue

2

Start / End Page

214 / 223

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Random Allocation
  • Quercetin
  • Nutrition & Dietetics
  • Male
  • Magnetic Resonance Spectroscopy
  • Lung
  • Lipopolysaccharides
  • HSP70 Heat-Shock Proteins
 

Citation

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Singleton, K. D., Serkova, N., Banerjee, A., Meng, X., Gamboni-Robertson, F., & Wischmeyer, P. E. (2005). Glutamine attenuates endotoxin-induced lung metabolic dysfunction: potential role of enhanced heat shock protein 70. Nutrition, 21(2), 214–223. https://doi.org/10.1016/j.nut.2004.05.023
Singleton, Kristen D., Natalie Serkova, Anirban Banerjee, Xianzhong Meng, Fabia Gamboni-Robertson, and Paul E. Wischmeyer. “Glutamine attenuates endotoxin-induced lung metabolic dysfunction: potential role of enhanced heat shock protein 70.Nutrition 21, no. 2 (February 2005): 214–23. https://doi.org/10.1016/j.nut.2004.05.023.
Singleton KD, Serkova N, Banerjee A, Meng X, Gamboni-Robertson F, Wischmeyer PE. Glutamine attenuates endotoxin-induced lung metabolic dysfunction: potential role of enhanced heat shock protein 70. Nutrition. 2005 Feb;21(2):214–23.
Singleton, Kristen D., et al. “Glutamine attenuates endotoxin-induced lung metabolic dysfunction: potential role of enhanced heat shock protein 70.Nutrition, vol. 21, no. 2, Feb. 2005, pp. 214–23. Pubmed, doi:10.1016/j.nut.2004.05.023.
Singleton KD, Serkova N, Banerjee A, Meng X, Gamboni-Robertson F, Wischmeyer PE. Glutamine attenuates endotoxin-induced lung metabolic dysfunction: potential role of enhanced heat shock protein 70. Nutrition. 2005 Feb;21(2):214–223.
Journal cover image

Published In

Nutrition

DOI

ISSN

0899-9007

Publication Date

February 2005

Volume

21

Issue

2

Start / End Page

214 / 223

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Random Allocation
  • Quercetin
  • Nutrition & Dietetics
  • Male
  • Magnetic Resonance Spectroscopy
  • Lung
  • Lipopolysaccharides
  • HSP70 Heat-Shock Proteins