Production of cloned goats from enucleated oocytes injected with cumulus cell nuclei or fused with cumulus cells.

Published

Journal Article

This study was designed to produce cloned goats from cumulus cells. Cloning donor nuclei were from cumulus cells either freshly isolated or cultured in vitro. Enucleated oocytes were either injected with cumulus cell nuclei without piezo-driven manipulator (injection method) or fused with cumulus cells (fusion method). The survival rate of cloned embryos, obtained by injection, was higher than that derived from fusion (62.7 and 45.9%, respectively). Two cloned goats were derived by fusion with in vitro cultured cumulus cells without starvation, but died shortly after natural birth, from respiratory difficulties. Their birth weights (2.23 kg and 2.03 kg) were within the normal range (2.0-2.7 kg) and postmortem analysis revealed no morphological abnormalities. The third cloned goat, derived by injection of nuclei from freshly isolated cumulus cells, weighed 3.3 kg at birth, and was 37% overweight compared with the average weight of the same species. This goat is healthy and well as this paper is being prepared. Nested PCR-RFLP analysis confirmed that all the cloned goats were derived from the donor cells.

Full Text

Cited Authors

  • Zou, X; Chen, Y; Wang, Y; Luo, J; Zhang, Q; Zhang, X; Yang, Y; Ju, H; Shen, Y; Lao, W; Xu, S; Du, M

Published Date

  • January 2001

Published In

Volume / Issue

  • 3 / 1

Start / End Page

  • 31 - 37

PubMed ID

  • 11918840

Pubmed Central ID

  • 11918840

International Standard Serial Number (ISSN)

  • 1520-4553

Digital Object Identifier (DOI)

  • 10.1089/152045501300189312

Language

  • eng