Gelatin-embedded cell-polymer constructs for histological cryosectioning.

Published

Journal Article

Many tissue-engineering strategies involve the delivery of cells via porous polymer scaffolds. Obtaining histological sections of the emerging tissue is often necessary to analyze numerous characteristics of the microscopic environment. However, difficulties arise upon applying standard histological techniques to cell-seeded polymer scaffolds. This report describes a simple and reliable method for cryosectioning cell-polymer constructs embedded in gelatin. Solvent-soluble (PLGA) and insoluble (PGA) scaffolds were cultured in vitro with preosteoblasts, followed by histological processing with paraffin, OCT, or gelatin. Although paraffin-embedded PGA scaffolds withstood standard sectioning and rinsing steps, paraffin-embedded PLGA scaffolds were partially dissolved during the clearing step. OCT-embedded scaffolds produced sections that did not adhere well to slides, and most of the sample was lost during rinsing steps. In contrast, gelatin-embedded scaffolds exhibited adequate structural integrity during cryosectioning, adhered well to the slides, retained the actual polymer morphology, and exhibited compatibility with common stains.

Full Text

Duke Authors

Cited Authors

  • Brown, DA; Chou, YF; Beygui, RE; Dunn, JCY; Wu, BM

Published Date

  • January 15, 2005

Published In

Volume / Issue

  • 72 / 1

Start / End Page

  • 79 - 85

PubMed ID

  • 15389500

Pubmed Central ID

  • 15389500

International Standard Serial Number (ISSN)

  • 1552-4973

Digital Object Identifier (DOI)

  • 10.1002/jbm.b.30116

Language

  • eng

Conference Location

  • United States