Skip to main content
Journal cover image

Modulation of gene expression in neonatal rat cardiomyocytes by surface modification of polylactide-co-glycolide substrates.

Publication ,  Journal Article
Brown, DA; Beygui, RE; MacLellan, WR; Laks, H; Dunn, JCY; Wu, BM
Published in: J Biomed Mater Res A
September 1, 2005

Myocardial tissue engineering presents a potential treatment option for heart disease. Cardiomyocytes isolated at various stages of development retain the ability to form contractile networks in vitro, which suggests that it should be possible to reconstitute viable myocardium given the appropriate architecture, stimuli, and cardiomyogenic cell source. This study investigates the effects of modifying substrate surface energy (by plasma etching) and protein coating (by fibronectin adsorption) on neonatal rat ventricular myocyte (NRVM) function. Primary NRVMs were cultured for 96 h on modified and control films of a common degradable polymer, polylactide-co-glycolide. Cultures were analyzed for cell spreading, protein content, and mRNA expression of atrial natriuretic factor and beta-myosin heavy chain. The results demonstrate that NRVMs cultured on etched films significantly increased in spreading, myofibril development, protein content, and gene expression of atrial natriuretic factor and beta-myosin heavy chain compared with unetched films, and that this surface energy effect is overwhelmed by the addition of fibronectin. Conclusions from this study are that surface energy and protein adsorption influence the gene expression of adherent NRVMs, and may be important for modulating the function of engineered myocardium.

Duke Scholars

Published In

J Biomed Mater Res A

DOI

ISSN

1549-3296

Publication Date

September 1, 2005

Volume

74

Issue

3

Start / End Page

419 / 429

Location

United States

Related Subject Headings

  • Wettability
  • Rats
  • Polyglactin 910
  • Myosin Heavy Chains
  • Myocytes, Cardiac
  • Gene Expression Regulation
  • Extracellular Matrix
  • Cells, Cultured
  • Biocompatible Materials
  • Atrial Natriuretic Factor
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Brown, D. A., Beygui, R. E., MacLellan, W. R., Laks, H., Dunn, J. C. Y., & Wu, B. M. (2005). Modulation of gene expression in neonatal rat cardiomyocytes by surface modification of polylactide-co-glycolide substrates. J Biomed Mater Res A, 74(3), 419–429. https://doi.org/10.1002/jbm.a.30344
Brown, David A., Ramin E. Beygui, William R. MacLellan, Hillel Laks, James C. Y. Dunn, and Benjamin M. Wu. “Modulation of gene expression in neonatal rat cardiomyocytes by surface modification of polylactide-co-glycolide substrates.J Biomed Mater Res A 74, no. 3 (September 1, 2005): 419–29. https://doi.org/10.1002/jbm.a.30344.
Brown DA, Beygui RE, MacLellan WR, Laks H, Dunn JCY, Wu BM. Modulation of gene expression in neonatal rat cardiomyocytes by surface modification of polylactide-co-glycolide substrates. J Biomed Mater Res A. 2005 Sep 1;74(3):419–29.
Brown, David A., et al. “Modulation of gene expression in neonatal rat cardiomyocytes by surface modification of polylactide-co-glycolide substrates.J Biomed Mater Res A, vol. 74, no. 3, Sept. 2005, pp. 419–29. Pubmed, doi:10.1002/jbm.a.30344.
Brown DA, Beygui RE, MacLellan WR, Laks H, Dunn JCY, Wu BM. Modulation of gene expression in neonatal rat cardiomyocytes by surface modification of polylactide-co-glycolide substrates. J Biomed Mater Res A. 2005 Sep 1;74(3):419–429.
Journal cover image

Published In

J Biomed Mater Res A

DOI

ISSN

1549-3296

Publication Date

September 1, 2005

Volume

74

Issue

3

Start / End Page

419 / 429

Location

United States

Related Subject Headings

  • Wettability
  • Rats
  • Polyglactin 910
  • Myosin Heavy Chains
  • Myocytes, Cardiac
  • Gene Expression Regulation
  • Extracellular Matrix
  • Cells, Cultured
  • Biocompatible Materials
  • Atrial Natriuretic Factor