Clonal sequences recovered from plasma from patients with residual HIV-1 viremia and on intensified antiretroviral therapy are identical to replicating viral RNAs recovered from circulating resting CD4+ T cells.
Journal Article (Journal Article)
Despite successful antiretroviral therapy (ART), low-level viremia (LLV) may be intermittently detected in most HIV-infected patients. Longitudinal blood plasma and resting CD4(+) T cells were obtained from two patients on suppressive ART to investigate the source of LLV. Single-genome sequencing of HIV-1 env from LLV plasma was performed, and the sequences were compared to sequences recovered from limiting-dilution outgrowth assays of resting CD4(+) T cells. The circulating LLV virus clone was identical to virus recovered from outgrowth assays from pools of millions of resting CD4(+) T cells. Understanding the sources of LLV requires evaluation of all possible reservoirs of persistent HIV infection.
Full Text
Duke Authors
Cited Authors
- Anderson, JA; Archin, NM; Ince, W; Parker, D; Wiegand, A; Coffin, JM; Kuruc, J; Eron, J; Swanstrom, R; Margolis, DM
Published Date
- May 2011
Published In
Volume / Issue
- 85 / 10
Start / End Page
- 5220 - 5223
PubMed ID
- 21367910
Pubmed Central ID
- PMC3126162
Electronic International Standard Serial Number (EISSN)
- 1098-5514
Digital Object Identifier (DOI)
- 10.1128/JVI.00284-11
Language
- eng
Conference Location
- United States