Clonal sequences recovered from plasma from patients with residual HIV-1 viremia and on intensified antiretroviral therapy are identical to replicating viral RNAs recovered from circulating resting CD4+ T cells.

Journal Article (Journal Article)

Despite successful antiretroviral therapy (ART), low-level viremia (LLV) may be intermittently detected in most HIV-infected patients. Longitudinal blood plasma and resting CD4(+) T cells were obtained from two patients on suppressive ART to investigate the source of LLV. Single-genome sequencing of HIV-1 env from LLV plasma was performed, and the sequences were compared to sequences recovered from limiting-dilution outgrowth assays of resting CD4(+) T cells. The circulating LLV virus clone was identical to virus recovered from outgrowth assays from pools of millions of resting CD4(+) T cells. Understanding the sources of LLV requires evaluation of all possible reservoirs of persistent HIV infection.

Full Text

Duke Authors

Cited Authors

  • Anderson, JA; Archin, NM; Ince, W; Parker, D; Wiegand, A; Coffin, JM; Kuruc, J; Eron, J; Swanstrom, R; Margolis, DM

Published Date

  • May 2011

Published In

Volume / Issue

  • 85 / 10

Start / End Page

  • 5220 - 5223

PubMed ID

  • 21367910

Pubmed Central ID

  • PMC3126162

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

Digital Object Identifier (DOI)

  • 10.1128/JVI.00284-11

Language

  • eng

Conference Location

  • United States