Suppression subtractive hybridization: A new method for cDNA subtraction of differentially expressed genes

A new and highly effective method, termed Suppression Subtractive Hybridization (SSH), has been developed for cDNA subtraction of differentially expressed genes, including the generation of subtracted cDNA libraries. It is based primarily on a recently described technique called suppression PCR and combines normalization and subtraction in a single procedure. The normalization step equalizes the abundance of cDNAs within the target population and the subtraction step excludes the common sequences between the target and driver populations. In a model system, the SSH technique enriched for rare sequences over 1,000-fold in one round of subtractive hybridization. We demonstrate its usefulness by generating a testisspecific cDNA library and by using the subtracted cDNA mixture as a hybridization probe to identify homologous sequences in a human Y chromosome cosmid library. The human DNA inserts in the isolated cosmids were further confirmed to be expressed in a testis-specific manner. These results suggest that the SSH technique is applicable to many molecular genetic and positional cloning studies for the identification of disease, developmental, cancer-related or other differentially expressed genes.

Duke Scholars

Author Luda Diatchenko Anesthesiology