Cellular Sites and Mechanisms Linking Reduction of Dipeptidyl Peptidase-4 Activity to Control of Incretin Hormone Action and Glucose Homeostasis.

Published

Journal Article

Pharmacological inhibition of the dipeptidyl peptidase-4 (DPP4) enzyme potentiates incretin action and is widely used to treat type 2 diabetes. Nevertheless, the precise cells and tissues critical for incretin degradation and glucose homeostasis remain unknown. Here, we use mouse genetics and pharmacologic DPP4 inhibition to identify DPP4+ cell types essential for incretin action. Although enterocyte DPP4 accounted for substantial intestinal DPP4 activity, ablation of enterocyte DPP4 in Dpp4Gut-/- mice did not produce alterations in plasma DPP4 activity, incretin hormone levels, and glucose tolerance. In contrast, endothelial cell (EC)-derived DPP4 contributed substantially to levels of soluble plasma DPP4 activity, incretin degradation, and glucose control. Surprisingly, DPP4+ cells of bone marrow origin mediated the selective degradation of fasting GIP, but not GLP-1. Collectively, these findings identify distinct roles for DPP4 in the EC versus the bone marrow compartment for selective incretin degradation and DPP4i-mediated glucoregulation.

Full Text

Duke Authors

Cited Authors

  • Mulvihill, EE; Varin, EM; Gladanac, B; Campbell, JE; Ussher, JR; Baggio, LL; Yusta, B; Ayala, J; Burmeister, MA; Matthews, D; Bang, KWA; Ayala, JE; Drucker, DJ

Published Date

  • January 10, 2017

Published In

Volume / Issue

  • 25 / 1

Start / End Page

  • 152 - 165

PubMed ID

  • 27839908

Pubmed Central ID

  • 27839908

Electronic International Standard Serial Number (EISSN)

  • 1932-7420

Digital Object Identifier (DOI)

  • 10.1016/j.cmet.2016.10.007

Language

  • eng

Conference Location

  • United States