Effect of fasting and feeding on measurement of carbon dioxide production using doubly labeled water.

Journal Article (Journal Article)

The doubly labeled water method is a noninvasive technique for measurement of rates of CO2 production and total energy expenditure in free-living human subjects. The experimental protocol used in validation studies usually involves prolonged fasting before and after the isotope dose is given to start the study, although it is not clear whether this intrusive aspect of the method is necessary. We investigated this issue in four healthy adults [3 women and 1 man: age 29 +/- 2 (SD) yr, body mass index 22 +/- 2.7 kg.m2] with monitored constant physical activity who underwent two doubly labeled water studies that differed only in the duration of fasting before and after isotope dosing (either 6 h of fasting before and 5 h after dosing or 0.5 h before and 3 h afterward). No significant difference between the two measurements was found in the rate constants for isotope disappearance, the ratio of the dilution spaces of the isotopes, or CO2 production rate. These results indicate that prolonged fasting before and after isotope administration should not be necessary in doubly labeled water studies.

Full Text

Duke Authors

Cited Authors

  • Calazel, CM; Young, VR; Evans, WJ; Roberts, SB

Published Date

  • April 1993

Published In

Volume / Issue

  • 74 / 4

Start / End Page

  • 1824 - 1829

PubMed ID

  • 8390442

International Standard Serial Number (ISSN)

  • 8750-7587

Digital Object Identifier (DOI)

  • 10.1152/jappl.1993.74.4.1824


  • eng

Conference Location

  • United States