Lipids Reprogram Metabolism to Become a Major Carbon Source for Histone Acetylation.

Journal Article (Journal Article)

Cells integrate nutrient sensing and metabolism to coordinate proper cellular responses to a particular nutrient source. For example, glucose drives a gene expression program characterized by activating genes involved in its metabolism, in part by increasing glucose-derived histone acetylation. Here, we find that lipid-derived acetyl-CoA is a major source of carbon for histone acetylation. Using 13C-carbon tracing combined with acetyl-proteomics, we show that up to 90% of acetylation on certain histone lysines can be derived from fatty acid carbon, even in the presence of excess glucose. By repressing both glucose and glutamine metabolism, fatty acid oxidation reprograms cellular metabolism, leading to increased lipid-derived acetyl-CoA. Gene expression profiling of octanoate-treated hepatocytes shows a pattern of upregulated lipid metabolic genes, demonstrating a specific transcriptional response to lipid. These studies expand the landscape of nutrient sensing and uncover how lipids and metabolism are integrated by epigenetic events that control gene expression.

Full Text

Duke Authors

Cited Authors

  • McDonnell, E; Crown, SB; Fox, DB; Kitir, B; Ilkayeva, OR; Olsen, CA; Grimsrud, PA; Hirschey, MD

Published Date

  • November 1, 2016

Published In

Volume / Issue

  • 17 / 6

Start / End Page

  • 1463 - 1472

PubMed ID

  • 27806287

Pubmed Central ID

  • PMC5123807

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2016.10.012


  • eng

Conference Location

  • United States