p53 Regulates Progenitor Cell Quiescence and Differentiation in the Airway.

Journal Article (Journal Article)

Mechanisms that regulate progenitor cell quiescence and differentiation in slowly replacing tissues are not fully understood. Here, we demonstrate that the tumor suppressor p53 regulates both proliferation and differentiation of progenitors in the airway epithelium. p53 loss decreased ciliated cell differentiation and increased the self-renewal and proliferative capacity of club progenitors, increasing epithelial cell density. p53-deficient progenitors generated a pseudostratified epithelium containing basal-like cells in vitro and putative bronchioalveolar stem cells in vivo. Conversely, an additional copy of p53 increased quiescence and ciliated cell differentiation, highlighting the importance of tight regulation of p53 levels. Using single-cell RNA sequencing, we found that loss of p53 altered the molecular phenotype of progenitors and differentially modulated cell-cycle regulatory genes. Together, these findings reveal that p53 is an essential regulator of progenitor cell behavior, which shapes our understanding of stem cell quiescence during homeostasis and in cancer development.

Full Text

Duke Authors

Cited Authors

  • McConnell, AM; Yao, C; Yeckes, AR; Wang, Y; Selvaggio, AS; Tang, J; Kirsch, DG; Stripp, BR

Published Date

  • November 22, 2016

Published In

Volume / Issue

  • 17 / 9

Start / End Page

  • 2173 - 2182

PubMed ID

  • 27880895

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2016.11.007


  • eng

Conference Location

  • United States