Capturing and Manipulating Activated Neuronal Ensembles with CANE Delineates a Hypothalamic Social-Fear Circuit.
Journal Article (Journal Article)
We developed a technology (capturing activated neuronal ensembles [CANE]) to label, manipulate, and transsynaptically trace neural circuits that are transiently activated in behavioral contexts with high efficiency and temporal precision. CANE consists of a knockin mouse and engineered viruses designed to specifically infect activated neurons. Using CANE, we selectively labeled neurons that were activated by either fearful or aggressive social encounters in a hypothalamic subnucleus previously known as a locus for aggression, and discovered that social-fear and aggression neurons are intermixed but largely distinct. Optogenetic stimulation of CANE-captured social-fear neurons (SFNs) is sufficient to evoke fear-like behaviors in normal social contexts, whereas silencing SFNs resulted in reduced social avoidance. CANE-based mapping of axonal projections and presynaptic inputs to SFNs further revealed a highly distributed and recurrent neural network. CANE is a broadly applicable technology for dissecting causality and connectivity of spatially intermingled but functionally distinct ensembles.
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Duke Authors
Cited Authors
- Sakurai, K; Zhao, S; Takatoh, J; Rodriguez, E; Lu, J; Leavitt, AD; Fu, M; Han, B-X; Wang, F
Published Date
- November 23, 2016
Published In
Volume / Issue
- 92 / 4
Start / End Page
- 739 - 753
PubMed ID
- 27974160
Pubmed Central ID
- PMC5172402
Electronic International Standard Serial Number (EISSN)
- 1097-4199
Digital Object Identifier (DOI)
- 10.1016/j.neuron.2016.10.015
Language
- eng
Conference Location
- United States