Loss of Bladder Epithelium Induced by Cytolytic Mast Cell Granules.


Journal Article

Programmed death and shedding of epithelial cells is a powerful defense mechanism to reduce bacterial burden during infection but this activity cannot be indiscriminate because of the critical barrier function of the epithelium. We report that during cystitis, shedding of infected bladder epithelial cells (BECs) was preceded by the recruitment of mast cells (MCs) directly underneath the superficial epithelium where they docked and extruded their granules. MCs were responding to interleukin-1β (IL-1β) secreted by BECs after inflammasome and caspase-1 signaling. Upon uptake of granule-associated chymase (mouse MC protease 4 [mMCPT4]), BECs underwent caspase-1-associated cytolysis and exfoliation. Thus, infected epithelial cells require a specific cue for cytolysis from recruited sentinel inflammatory cells before shedding.

Full Text

Duke Authors

Cited Authors

  • Choi, HW; Bowen, SE; Miao, Y; Chan, CY; Miao, EA; Abrink, M; Moeser, AJ; Abraham, SN

Published Date

  • December 20, 2016

Published In

Volume / Issue

  • 45 / 6

Start / End Page

  • 1258 - 1269

PubMed ID

  • 27939674

Pubmed Central ID

  • 27939674

Electronic International Standard Serial Number (EISSN)

  • 1097-4180

Digital Object Identifier (DOI)

  • 10.1016/j.immuni.2016.11.003


  • eng

Conference Location

  • United States