Divergent regulation of functionally distinct γ-tubulin complexes during differentiation.
Journal Article (Journal Article)
Differentiation induces the formation of noncentrosomal microtubule arrays in diverse tissues. The formation of these arrays requires loss of microtubule-organizing activity (MTOC) at the centrosome, but the mechanisms regulating this transition remain largely unexplored. Here, we use the robust loss of centrosomal MTOC activity in the epidermis to identify two pools of γ-tubulin that are biochemically and functionally distinct and differentially regulated. Nucleation-competent CDK5RAP2-γ-tubulin complexes were maintained at centrosomes upon initial epidermal differentiation. In contrast, Nedd1-γ-tubulin complexes did not promote nucleation but were required for anchoring of microtubules, a previously uncharacterized activity for this complex. Cell cycle exit specifically triggered loss of Nedd1-γ-tubulin complexes, providing a mechanistic link connecting MTOC activity and differentiation. Collectively, our studies demonstrate that distinct γ-tubulin complexes regulate different microtubule behaviors at the centrosome and show that differential regulation of these complexes drives loss of centrosomal MTOC activity.
Full Text
Duke Authors
Cited Authors
- Muroyama, A; Seldin, L; Lechler, T
Published Date
- June 20, 2016
Published In
Volume / Issue
- 213 / 6
Start / End Page
- 679 - 692
PubMed ID
- 27298324
Pubmed Central ID
- PMC4915192
Electronic International Standard Serial Number (EISSN)
- 1540-8140
Digital Object Identifier (DOI)
- 10.1083/jcb.201601099
Language
- eng
Conference Location
- United States