Optical detection of molecular beacons in microfluidic devices
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, Conference
Balberg, M; Hristova, R; Brady, DJ; Beebe, DJ; Raskin, L
Published in: 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
January 1, 2000
Hybridization and detection of E. coli ribosomal RNA with molecular beacons labeled with a fluorescent dye Is achieved in a microfluidic device. The device consists of a four ports mixing chamber, where the probes and the target molecules mix by diffusion. The weak fluorescent signal is detected by two large core optical fibers that are placed on both sides of the channel and collect the light emitted in both directions. The fibers are coupled to a spectrophotometer. The detection limit for the system is 0.2 ftnol.
Duke Scholars
Published In
1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
DOI
ISBN
9780780366039
Publication Date
January 1, 2000
Start / End Page
425 / 428
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Balberg, M., Hristova, R., Brady, D. J., Beebe, D. J., & Raskin, L. (2000). Optical detection of molecular beacons in microfluidic devices. In 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings (pp. 425–428). https://doi.org/10.1109/MMB.2000.893820
Balberg, M., R. Hristova, D. J. Brady, D. J. Beebe, and L. Raskin. “Optical detection of molecular beacons in microfluidic devices.” In 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings, 425–28, 2000. https://doi.org/10.1109/MMB.2000.893820.
Balberg M, Hristova R, Brady DJ, Beebe DJ, Raskin L. Optical detection of molecular beacons in microfluidic devices. In: 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. 2000. p. 425–8.
Balberg, M., et al. “Optical detection of molecular beacons in microfluidic devices.” 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings, 2000, pp. 425–28. Scopus, doi:10.1109/MMB.2000.893820.
Balberg M, Hristova R, Brady DJ, Beebe DJ, Raskin L. Optical detection of molecular beacons in microfluidic devices. 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. 2000. p. 425–428.
Published In
1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
DOI
ISBN
9780780366039
Publication Date
January 1, 2000
Start / End Page
425 / 428