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Cyclocreatine inhibits stimulated motility in tumor cells possessing creatine kinase.

Publication ,  Journal Article
Mulvaney, PT; Stracke, ML; Nam, SW; Woodhouse, E; O'Keefe, M; Clair, T; Liotta, LA; Khaddurah-Daouk, R; Schiffmann, E
Published in: Int J Cancer
September 25, 1998

Cyclocreatine (1-carboxymethyl-2-iminoimidazolidine), an analog of creatine and a substrate for creatine kinase (EC 2.7.3.2), inhibits the stimulated motility of tumor cells which possess creatine kinase. A2058-055 human melanoma cells, transfected with a creatine kinase gene, showed an 80-90% reduction in chemotactic response to type IV collagen when incubated overnight in the presence of 10 mM cyclocreatine (p < 0.0001 for n = 8 experiments). This inhibitory effect of cyclocreatine can be partially reversed by addition of creatine to the overnight cell treatment. Non-transfected cells, with very low levels of creatine kinase, were not significantly inhibited. Further experiments utilizing type IV collagen as attractant demonstrated that cyclocreatine inhibited the chemokinetic (91%) and the haptotactic (73%) responses and the in vitro invasion of A2058-055 cells through Matrigel-coated membranes (88%). In addition, motility stimulation of A2058-055 cells by either autotaxin or fibronectin was markedly inhibited by cyclocreatine. DU-145 prostatic tumor cells, which express endogenous creatine kinase, also have a reduced motility response to either autotaxin or epidermal growth factor induced motility in the presence of cyclocreatine.

Duke Scholars

Published In

Int J Cancer

DOI

ISSN

0020-7136

Publication Date

September 25, 1998

Volume

78

Issue

1

Start / End Page

46 / 52

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Prostatic Neoplasms
  • Oncology & Carcinogenesis
  • Neoplasm Invasiveness
  • Melanoma
  • Male
  • Humans
  • Creatinine
  • Creatine Kinase
  • Creatine
 

Citation

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Mulvaney, P. T., Stracke, M. L., Nam, S. W., Woodhouse, E., O’Keefe, M., Clair, T., … Schiffmann, E. (1998). Cyclocreatine inhibits stimulated motility in tumor cells possessing creatine kinase. Int J Cancer, 78(1), 46–52. https://doi.org/10.1002/(sici)1097-0215(19980925)78:1<46::aid-ijc9>3.0.co;2-x
Mulvaney, P. T., M. L. Stracke, S. W. Nam, E. Woodhouse, M. O’Keefe, T. Clair, L. A. Liotta, R. Khaddurah-Daouk, and E. Schiffmann. “Cyclocreatine inhibits stimulated motility in tumor cells possessing creatine kinase.Int J Cancer 78, no. 1 (September 25, 1998): 46–52. https://doi.org/10.1002/(sici)1097-0215(19980925)78:1<46::aid-ijc9>3.0.co;2-x.
Mulvaney PT, Stracke ML, Nam SW, Woodhouse E, O’Keefe M, Clair T, et al. Cyclocreatine inhibits stimulated motility in tumor cells possessing creatine kinase. Int J Cancer. 1998 Sep 25;78(1):46–52.
Mulvaney, P. T., et al. “Cyclocreatine inhibits stimulated motility in tumor cells possessing creatine kinase.Int J Cancer, vol. 78, no. 1, Sept. 1998, pp. 46–52. Pubmed, doi:10.1002/(sici)1097-0215(19980925)78:1<46::aid-ijc9>3.0.co;2-x.
Mulvaney PT, Stracke ML, Nam SW, Woodhouse E, O’Keefe M, Clair T, Liotta LA, Khaddurah-Daouk R, Schiffmann E. Cyclocreatine inhibits stimulated motility in tumor cells possessing creatine kinase. Int J Cancer. 1998 Sep 25;78(1):46–52.
Journal cover image

Published In

Int J Cancer

DOI

ISSN

0020-7136

Publication Date

September 25, 1998

Volume

78

Issue

1

Start / End Page

46 / 52

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Prostatic Neoplasms
  • Oncology & Carcinogenesis
  • Neoplasm Invasiveness
  • Melanoma
  • Male
  • Humans
  • Creatinine
  • Creatine Kinase
  • Creatine