Signal-on Protein Detection via Dye Translocation between Aptamer and Quantum Dot.

Published

Journal Article

A unique interaction between the cyanine dye and negatively charged quantum dot is used to construct a signal-on biaptameric quantum dot (QD) Förster resonance energy transfer (FRET) beacon for protein detection and distinct aptamer characterization. The beacon comprises a pair of aptamers, one intercalated with the cyanine dye (YOYO-3) and the other conjugated to a negatively charged, carboxyl-QD. When the target protein is present, structural folding and sandwich association of the two aptamers take place. As a consequence, YOYO-3 is displaced from the folded aptamer and transferred to the unblocked QD surface to yield a target concentration-dependent FRET signal. As a proof-of-principle, we demonstrate the detection of thrombin ranging from nanomolar to submicromolar concentrations and confirm the dye translocation using cylindrical illumination confocal spectroscopy (CICS). The proposed beacon provides a simple, rapid, signal-on FRET detection for protein as well as a potential platform for distinct aptamer screening.

Full Text

Cited Authors

  • Lao, Y-H; Chi, C-W; Friedrich, SM; Peck, K; Wang, T-H; Leong, KW; Chen, L-C

Published Date

  • May 3, 2016

Published In

Volume / Issue

  • 8 / 19

Start / End Page

  • 12048 - 12055

PubMed ID

  • 27101438

Pubmed Central ID

  • 27101438

Electronic International Standard Serial Number (EISSN)

  • 1944-8252

International Standard Serial Number (ISSN)

  • 1944-8244

Digital Object Identifier (DOI)

  • 10.1021/acsami.6b02871

Language

  • eng