Phosphatidylcholine metabolism in lung microsomes and lung surfactant of rats exposed intratracheally to coal fly ash.

Journal Article (Journal Article)

The effect of intratracheal administration of fly ash has been studied on lung microsomal and lung surfactant phosphatidylcholine (PC) metabolism in rats using [methyl-14C]choline and [methyl-14C]methionine. Fly-ash administration significantly increased total phospholipids, PC, phosphatidylethanolamine (PE), and phosphatidylglycerol (PG) of lung surfactant. Fly-ash administration stimulated the formation of lung microsomal PC (as measured by the incorporation of labeled precursors) both by the cytidine 5'-diphosphate (CDP)-choline pathway and by the N-methylation pathway, but this stimulation was fourfold higher in the latter case and only twofold higher in the former as compared to the control. Likewise, the secretion of PC formed by the N-methylation pathway was sixfold higher as compared to the control whereas secretion of PC formed by the CDP-choline pathway was only threefold higher as compared to the control. Fly-ash administration further increased total saturation and decreased unsaturation in PC, PE, and lysophosphatidylcholine (LPC) of lung and in PC, PE, LPC, and PG of lung surfactant as compared to the controls.

Full Text

Duke Authors

Cited Authors

  • Srivastava, PK; Misra, UK

Published Date

  • 1986

Published In

Volume / Issue

  • 18 / 3

Start / End Page

  • 471 - 481

PubMed ID

  • 3754906

International Standard Serial Number (ISSN)

  • 0098-4108

Digital Object Identifier (DOI)

  • 10.1080/15287398609530886


  • eng

Conference Location

  • United States