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Phosphoinositides of sheep platelets plasma membranes.

Publication ,  Journal Article
Kumar, V; Srivastava, N; Grover, SK; Jaiswal, AS; Bansal, SK; Misra, UK
Published in: Indian J Biochem Biophys
April 1991

Phospholipid composition of sheep blood platelets and its various plasma membrane fractions have been analyzed. Based on their flotation rates in discontinuous sucrose density gradient centrifugation, three membrane fractions were isolated. 5'-Nucelotidase and alkaline phosphatase were distributed nearly equally in all the three membrane fractions. However these membrane fractions showed differences in the distribution of phosphatidyl ethanolamine, phosphatidyl choline and phosphoinositides. Phosphatidyl ethanolamine was predominant in fraction I (11.05 micrograms PLP/mg protein) while phosphatidyl choline was predominant in fractions II and III (110.10 and 68.30 micrograms PLP/mg protein respectively). Phosphatidyl inositol (Ptd-InsP) was equally distributed in all three membrane fractions. However, both Ptd-InsP and phosphatidyl inositol 4,5-bisphosphate were about 4-fold higher in fraction II (73.55 and 89.89 micrograms PLP/mg protein respectively).

Duke Scholars

Published In

Indian J Biochem Biophys

ISSN

0301-1208

Publication Date

April 1991

Volume

28

Issue

2

Start / End Page

133 / 135

Location

India

Related Subject Headings

  • Sheep
  • Phospholipids
  • Phosphatidylinositols
  • Membrane Lipids
  • Centrifugation, Density Gradient
  • Cell Membrane
  • Cell Fractionation
  • Blood Platelets
  • Biochemistry & Molecular Biology
  • Animals
 

Citation

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Kumar, V., Srivastava, N., Grover, S. K., Jaiswal, A. S., Bansal, S. K., & Misra, U. K. (1991). Phosphoinositides of sheep platelets plasma membranes. Indian J Biochem Biophys, 28(2), 133–135.
Kumar, V., N. Srivastava, S. K. Grover, A. S. Jaiswal, S. K. Bansal, and U. K. Misra. “Phosphoinositides of sheep platelets plasma membranes.Indian J Biochem Biophys 28, no. 2 (April 1991): 133–35.
Kumar V, Srivastava N, Grover SK, Jaiswal AS, Bansal SK, Misra UK. Phosphoinositides of sheep platelets plasma membranes. Indian J Biochem Biophys. 1991 Apr;28(2):133–5.
Kumar, V., et al. “Phosphoinositides of sheep platelets plasma membranes.Indian J Biochem Biophys, vol. 28, no. 2, Apr. 1991, pp. 133–35.
Kumar V, Srivastava N, Grover SK, Jaiswal AS, Bansal SK, Misra UK. Phosphoinositides of sheep platelets plasma membranes. Indian J Biochem Biophys. 1991 Apr;28(2):133–135.

Published In

Indian J Biochem Biophys

ISSN

0301-1208

Publication Date

April 1991

Volume

28

Issue

2

Start / End Page

133 / 135

Location

India

Related Subject Headings

  • Sheep
  • Phospholipids
  • Phosphatidylinositols
  • Membrane Lipids
  • Centrifugation, Density Gradient
  • Cell Membrane
  • Cell Fractionation
  • Blood Platelets
  • Biochemistry & Molecular Biology
  • Animals