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Syndecans, cell surface heparan sulfate proteoglycans, are induced by a proline-rich antimicrobial peptide from wounds.

Publication ,  Journal Article
Gallo, RL; Ono, M; Povsic, T; Page, C; Eriksson, E; Klagsbrun, M; Bernfield, M
Published in: Proc Natl Acad Sci U S A
November 8, 1994

Cell surface heparan sulfate proteoglycans, such as the syndecans, are required for cellular responses to heparin-binding growth factors and extracellular matrix components. Expression of syndecan-1 and -4 is induced in mesenchymal cells during wound repair in the mouse, consistent with a role for syndecans in regulating cell proliferation and migration in response to these effectors. Here we show that wound fluid contains inductive activity that mimics the in vivo induction in time of appearance, specificity for mesenchymal cells, and selectivity for syndecan-1 and -4. We have purified and synthesized a 4.8-kDa proline-rich protein from wound fluid that reproduces this induction of syndecan-1 and -4 in cultured cells. This peptide, identical to the antibacterial peptide PR-39, is released into the wound by the cellular infiltrate and induces syndecan expression at the same peptide concentrations that lyse bacteria. These results indicate that wounds contain a multifunctional protein that induces mammalian cells to express cell surface heparan sulfate proteoglycans as part of the wound repair process and that kills bacteria as part of a nonimmune defense mechanism.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 8, 1994

Volume

91

Issue

23

Start / End Page

11035 / 11039

Location

United States

Related Subject Headings

  • Wound Healing
  • Syndecans
  • Syndecan-4
  • Syndecan-1
  • Swine
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • RNA, Messenger
  • Proteoglycans
  • Proline-Rich Protein Domains
 

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Gallo, R. L., Ono, M., Povsic, T., Page, C., Eriksson, E., Klagsbrun, M., & Bernfield, M. (1994). Syndecans, cell surface heparan sulfate proteoglycans, are induced by a proline-rich antimicrobial peptide from wounds. Proc Natl Acad Sci U S A, 91(23), 11035–11039. https://doi.org/10.1073/pnas.91.23.11035
Gallo, R. L., M. Ono, T. Povsic, C. Page, E. Eriksson, M. Klagsbrun, and M. Bernfield. “Syndecans, cell surface heparan sulfate proteoglycans, are induced by a proline-rich antimicrobial peptide from wounds.Proc Natl Acad Sci U S A 91, no. 23 (November 8, 1994): 11035–39. https://doi.org/10.1073/pnas.91.23.11035.
Gallo RL, Ono M, Povsic T, Page C, Eriksson E, Klagsbrun M, et al. Syndecans, cell surface heparan sulfate proteoglycans, are induced by a proline-rich antimicrobial peptide from wounds. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11035–9.
Gallo, R. L., et al. “Syndecans, cell surface heparan sulfate proteoglycans, are induced by a proline-rich antimicrobial peptide from wounds.Proc Natl Acad Sci U S A, vol. 91, no. 23, Nov. 1994, pp. 11035–39. Pubmed, doi:10.1073/pnas.91.23.11035.
Gallo RL, Ono M, Povsic T, Page C, Eriksson E, Klagsbrun M, Bernfield M. Syndecans, cell surface heparan sulfate proteoglycans, are induced by a proline-rich antimicrobial peptide from wounds. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11035–11039.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 8, 1994

Volume

91

Issue

23

Start / End Page

11035 / 11039

Location

United States

Related Subject Headings

  • Wound Healing
  • Syndecans
  • Syndecan-4
  • Syndecan-1
  • Swine
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • RNA, Messenger
  • Proteoglycans
  • Proline-Rich Protein Domains