Transiently expressed ATG16L1 inhibits autophagosome biogenesis and aberrantly targets RAB11-positive recycling endosomes.

Published

Journal Article

The membrane source for autophagosome biogenesis is an unsolved mystery in the study of autophagy. ATG16L1 forms a complex with ATG12-ATG5 (the ATG16L1 complex). The ATG16L1 complex is recruited to autophagic membranes to convert MAP1LC3B-I to MAP1LC3B-II. The ATG16L1 complex dissociates from the phagophore before autophagosome membrane closure. Thus, ATG16L1 can be used as an early event marker for the study of autophagosome biogenesis. We found that among 3 proteins in the ATG16L1 complex, only ATG16L1 formed puncta-like structures when transiently overexpressed. ATG16L1+ puncta formed by transient expression could represent autophagic membrane structures. We thoroughly characterized the transiently expressed ATG16L1 in several mammalian cell lines. We found that transient expression of ATG16L1 not only inhibited autophagosome biogenesis, but also aberrantly targeted RAB11-positive recycling endosomes, resulting in recycling endosome aggregates. We conclude that transient expression of ATG16L1 is not a physiological model for the study of autophagy. Caution is warranted when reviewing findings derived from a transient expression model of ATG16L1.

Full Text

Duke Authors

Cited Authors

  • Li, J; Chen, Z; Stang, MT; Gao, W

Published Date

  • February 2017

Published In

Volume / Issue

  • 13 / 2

Start / End Page

  • 345 - 358

PubMed ID

  • 27875067

Pubmed Central ID

  • 27875067

Electronic International Standard Serial Number (EISSN)

  • 1554-8635

Digital Object Identifier (DOI)

  • 10.1080/15548627.2016.1256521

Language

  • eng

Conference Location

  • United States