The Genetic Basis of Hepatosplenic T-cell Lymphoma.

Journal Article

Hepatosplenic T-cell lymphoma (HSTL) is a rare and lethal lymphoma; the genetic drivers of this disease are unknown. Through whole-exome sequencing of 68 HSTLs, we define recurrently mutated driver genes and copy-number alterations in the disease. Chromatin-modifying genes, including SETD2, INO80, and ARID1B, were commonly mutated in HSTL, affecting 62% of cases. HSTLs manifest frequent mutations in STAT5B (31%), STAT3 (9%), and PIK3CD (9%), for which there currently exist potential targeted therapies. In addition, we noted less frequent events in EZH2, KRAS, and TP53SETD2 was the most frequently silenced gene in HSTL. We experimentally demonstrated that SETD2 acts as a tumor suppressor gene. In addition, we found that mutations in STAT5B and PIK3CD activate critical signaling pathways important to cell survival in HSTL. Our work thus defines the genetic landscape of HSTL and implicates gene mutations linked to HSTL pathogenesis and potential treatment targets.Significance: We report the first systematic application of whole-exome sequencing to define the genetic basis of HSTL, a rare but lethal disease. Our work defines SETD2 as a tumor suppressor gene in HSTL and implicates genes including INO80 and PIK3CD in the disease. Cancer Discov; 7(4); 369-79. ©2017 AACR.See related commentary by Yoshida and Weinstock, p. 352This article is highlighted in the In This Issue feature, p. 339.

Full Text

Duke Authors

Cited Authors

  • McKinney, M; Moffitt, AB; Gaulard, P; Travert, M; De Leval, L; Nicolae, A; Raffeld, M; Jaffe, ES; Pittaluga, S; Xi, L; Heavican, T; Iqbal, J; Belhadj, K; Delfau-Larue, MH; Fataccioli, V; Czader, MB; Lossos, IS; Chapman-Fredricks, JR; Richards, KL; Fedoriw, Y; Ondrejka, SL; Hsi, ED; Low, L; Weisenburger, D; Chan, WC; Mehta-Shah, N; Horwitz, S; Bernal-Mizrachi, L; Flowers, CR; Beaven, AW; Parihar, M; Baseggio, L; Parrens, M; Moreau, A; Sujobert, P; Pilichowska, M; Evens, AM; Chadburn, A et al.

Published Date

  • April 2017

Published In

Volume / Issue

  • 7 / 4

Start / End Page

  • 369 - 379

PubMed ID

  • 28122867

Electronic International Standard Serial Number (EISSN)

  • 2159-8290

International Standard Serial Number (ISSN)

  • 2159-8274

Digital Object Identifier (DOI)

  • 10.1158/2159-8290.cd-16-0330

Language

  • eng