A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens.

Published

Journal Article

Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3'-untranslated region (3'-UTR). Removal of the 3'-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28.

Full Text

Duke Authors

Cited Authors

  • Li, C; Sako, Y; Imai, A; Nishiyama, T; Thompson, K; Kubo, M; Hiwatashi, Y; Kabeya, Y; Karlson, D; Wu, S-H; Ishikawa, M; Murata, T; Benfey, PN; Sato, Y; Tamada, Y; Hasebe, M

Published Date

  • January 27, 2017

Published In

Volume / Issue

  • 8 /

Start / End Page

  • 14242 -

PubMed ID

  • 28128346

Pubmed Central ID

  • 28128346

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/ncomms14242

Language

  • eng