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Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain.

Publication ,  Journal Article
Maul, RW; Sanders, LH; Lim, JB; Benitez, R; Sutton, MD
Published in: J Bacteriol
July 2007

The Escherichia coli dnaN159 allele encodes a mutant form of the beta-sliding clamp (beta159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. In addition, strains bearing the dnaN159 allele require functional Pol I for viability. We have utilized a combination of genetic and biochemical approaches to characterize the role(s) played by Pol I in the dnaN159 strain. Our findings indicate that elevated levels of Pol I partially suppress the temperature-sensitive growth phenotype of the dnaN159 strain. In addition, we demonstrate that the beta clamp stimulates the processivity of Pol I in vitro and that beta159 is impaired for this activity. The reduced ability of beta159 to stimulate Pol I in vitro correlates with our finding that single-stranded DNA (ssDNA) gap repair is impaired in the dnaN159 strain. Taken together, these results suggest that (i) the beta clamp-Pol I interaction may be important for proper Pol I function in vivo and (ii) in the absence of Pol I, ssDNA gaps may persist in the dnaN159 strain, leading to lethality of the dnaN159 DeltapolA strain.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

July 2007

Volume

189

Issue

13

Start / End Page

4688 / 4695

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Temperature
  • Sequence Analysis, DNA
  • Mutation
  • Microbiology
  • Microbial Viability
  • Escherichia coli Proteins
  • Escherichia coli
  • DNA-Directed DNA Polymerase
  • DNA, Single-Stranded
 

Citation

APA
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ICMJE
MLA
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Maul, R. W., Sanders, L. H., Lim, J. B., Benitez, R., & Sutton, M. D. (2007). Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain. J Bacteriol, 189(13), 4688–4695. https://doi.org/10.1128/JB.00476-07
Maul, Robert W., Laurie H. Sanders, James B. Lim, Rosemary Benitez, and Mark D. Sutton. “Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain.J Bacteriol 189, no. 13 (July 2007): 4688–95. https://doi.org/10.1128/JB.00476-07.
Maul RW, Sanders LH, Lim JB, Benitez R, Sutton MD. Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain. J Bacteriol. 2007 Jul;189(13):4688–95.
Maul, Robert W., et al. “Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain.J Bacteriol, vol. 189, no. 13, July 2007, pp. 4688–95. Pubmed, doi:10.1128/JB.00476-07.
Maul RW, Sanders LH, Lim JB, Benitez R, Sutton MD. Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain. J Bacteriol. 2007 Jul;189(13):4688–4695.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

July 2007

Volume

189

Issue

13

Start / End Page

4688 / 4695

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Temperature
  • Sequence Analysis, DNA
  • Mutation
  • Microbiology
  • Microbial Viability
  • Escherichia coli Proteins
  • Escherichia coli
  • DNA-Directed DNA Polymerase
  • DNA, Single-Stranded