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Reduced thermal hyperalgesia and enhanced peripheral nerve injury after hind paw inflammation in mice lacking the serotonin‐transporter

Publication ,  Journal Article
Palm, F; Mössner, R; Chen, Y; He, L; Gerlach, M; Bischofs, S; Riederer, P; Lesch, K; Sommer, C
Published in: European Journal of Pain
August 2008

Mice lacking the serotonin‐transporter (5‐HTT‐/‐ mice) develop reduced thermal hyperalgesia after nerve injury, concomitant with reduced serotonin (5‐HT) levels in nervous tissue. Here we investigated pain behaviour in 5‐HTT‐/‐ mice compared to their wild type littermates after hind paw inflammation induced by complete Freund's adjuvant (CFA). We used standard tests for pain behaviour, high performance liquid chromatography for measurement of 5‐HT, and immunohistochemistry of hind paw skin tissue and L5 dorsal root ganglia (DRG) to measure local inflammation and nerve injury. After intraplantar CFA injection, hyperalgesia to heat was attenuated in 5‐HTT‐/‐ mice compared to wild type mice. Their 5‐HT levels in nervous and adrenal tissue were reduced. An intraplantar injection of 5‐HT four days after CFA transiently brought withdrawal latencies of 5‐HTT‐/‐ mice down to the level of wild type mice, thus rescuing the phenotype and supporting the role of 5‐HT in the development of CFA‐induced thermal hyperalgesia. The density of intraepidermal nerve fibres in plantar skin after CFA injection was reduced to a higher degree in 5‐HTT‐/‐ mice than in wild type mice, suggesting greater peripheral nerve injury in the knock‐out mice during hind paw inflammation. Accordingly, a higher number of injured DRG neurons was identified by activating transcription factor 3 (ATF3) staining in 5‐HTT‐/‐ mice after CFA. We conclude that the phenotype of 5‐HTT‐/‐ mice leads to reduced inflammatory pain due to reduced tissue 5‐HT levels and to greater peripheral nerve injury after inflammation. Human variants of the 5‐HTT genotypes might be part of the factors determining the extent of nerve injury and hyperalgesia in inflammation.

Duke Scholars

Published In

European Journal of Pain

DOI

EISSN

1532-2149

ISSN

1090-3801

Publication Date

August 2008

Volume

12

Issue

6

Start / End Page

790 / 797

Publisher

Wiley

Related Subject Headings

  • Anesthesiology
  • 3214 Pharmacology and pharmaceutical sciences
  • 3209 Neurosciences
  • 3202 Clinical sciences
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 1109 Neurosciences
  • 1103 Clinical Sciences
 

Citation

APA
Chicago
ICMJE
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Palm, F., Mössner, R., Chen, Y., He, L., Gerlach, M., Bischofs, S., … Sommer, C. (2008). Reduced thermal hyperalgesia and enhanced peripheral nerve injury after hind paw inflammation in mice lacking the serotonin‐transporter. European Journal of Pain, 12(6), 790–797. https://doi.org/10.1016/j.ejpain.2007.11.009
Palm, Florian, Rainald Mössner, Yong Chen, Lan He, Manfred Gerlach, Stefan Bischofs, Peter Riederer, Klaus‐Peter Lesch, and Claudia Sommer. “Reduced thermal hyperalgesia and enhanced peripheral nerve injury after hind paw inflammation in mice lacking the serotonin‐transporter.” European Journal of Pain 12, no. 6 (August 2008): 790–97. https://doi.org/10.1016/j.ejpain.2007.11.009.
Palm F, Mössner R, Chen Y, He L, Gerlach M, Bischofs S, et al. Reduced thermal hyperalgesia and enhanced peripheral nerve injury after hind paw inflammation in mice lacking the serotonin‐transporter. European Journal of Pain. 2008 Aug;12(6):790–7.
Palm, Florian, et al. “Reduced thermal hyperalgesia and enhanced peripheral nerve injury after hind paw inflammation in mice lacking the serotonin‐transporter.” European Journal of Pain, vol. 12, no. 6, Wiley, Aug. 2008, pp. 790–97. Crossref, doi:10.1016/j.ejpain.2007.11.009.
Palm F, Mössner R, Chen Y, He L, Gerlach M, Bischofs S, Riederer P, Lesch K, Sommer C. Reduced thermal hyperalgesia and enhanced peripheral nerve injury after hind paw inflammation in mice lacking the serotonin‐transporter. European Journal of Pain. Wiley; 2008 Aug;12(6):790–797.
Journal cover image

Published In

European Journal of Pain

DOI

EISSN

1532-2149

ISSN

1090-3801

Publication Date

August 2008

Volume

12

Issue

6

Start / End Page

790 / 797

Publisher

Wiley

Related Subject Headings

  • Anesthesiology
  • 3214 Pharmacology and pharmaceutical sciences
  • 3209 Neurosciences
  • 3202 Clinical sciences
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 1109 Neurosciences
  • 1103 Clinical Sciences