Reactivity of avian RNA tumor viruses with lectins.

Published

Journal Article

The infectivity of avian RNA tumor viruses was inactivated to varying degrees by treatment with either concanavalin A (Con A) or phytohemagglutinin but not by treatment with wheat germ agglutinin. In general, leukosis viruses reacted preferentially with Con A, whereas sarcoma viruses showed more affinity for phytohemagglutinin. In a more extensive study with subgroup A of Prague Rous sarcoma virus (PR-A), the effect of inactivation by Con A could be specifically prevented by the addition of alpha-methyl-D-mannoside, alpha-methyl-D-glucoside, and N-acetyl-D-glucosamine. These sugars were also capable of eluting [3H]glucosamine-labeled material from disrupted PR-A virus, which was bound to a Con A-sepharose affinity column. A major viral glycoprotein recovered from the column had the same mobility as gp85 in polyacrylamide gel electrophoresis and could be immunoprecipitated with anti-gp85 antiserum. These results suggest that the material reacting with Con A is present on the gp85 component of the viral glycoprotein. The diversity in the reactivity of the glycoproteins of transforming and nontransforming viruses with plant lectins is discussed.

Full Text

Duke Authors

Cited Authors

  • Ishizaki, R; Bolognesi, DP

Published Date

  • January 1975

Published In

Volume / Issue

  • 17 / 1

Start / End Page

  • 132 - 139

PubMed ID

  • 173878

Pubmed Central ID

  • 173878

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States