Testing homogeneity of intra-run variance parameters in immunoassay.

Journal Article (Journal Article)

A common assumption in the analysis of immunoassay data is a similar pattern of within-run variation across runs of the assays. One makes this assumption without formal investigation of its validity, despite the widely acknowledged fact that accurate understanding of intra-run variation is critical to reliable calibration inference. We propose a simple procedure for a formal test of the assumption of the homogeneity of parameters that characterize intra-run variation based on representation of standard curve data from multiple assay runs by a non-linear mixed effects model. We examine the performance of the procedure and investigate the robustness of calibration inference to incorrect assumptions about the pattern of intra-run variation.

Full Text

Duke Authors

Cited Authors

  • Zeng, Q; Davidian, M

Published Date

  • August 15, 1997

Published In

Volume / Issue

  • 16 / 15

Start / End Page

  • 1765 - 1776

PubMed ID

  • 9265699

International Standard Serial Number (ISSN)

  • 0277-6715

Digital Object Identifier (DOI)

  • 10.1002/(sici)1097-0258(19970815)16:15<1765::aid-sim603>3.0.co;2-p


  • eng

Conference Location

  • England