Ibuprofen-mediated reversal of fluconazole resistance in clinical isolates of Candida.

Published

Journal Article

INTRODUCTION: In view of the increasing prevalence of invasive Candidiasis in today's health-care scenario and the emergence of fluconazole resistance among clinical isolates of Candida, we sought to determine if Ibuprofen could elicit a reversal of fluconazole resistance and thereby offer a potential therapeutic breakthrough in fluconazole-resistant Candidiasis. MATERIALS AND METHODS: We selected 69 clinical isolates of Candida, which demonstrated an MIC of >32 μg/ml for fluconazole, and subjected them to broth microdilution in presence and absence of Ibuprofen. RESULTS: Forty two of the 69 isolates (60.9%) demonstrated reversal of Fluconazole resistance with concomitant use of Ibuprofen. This was characterized by significant species-wise variation (p=0.00008), with all the C. albicans isolates and none of the C. glabrata isolates demonstrating such reversal. Only 22.2% and 37.7% of C. krusei and C. tropicalis isolates respectively showed Ibuprofen-mediated reversal of Fluconazole resistance. CONCLUSION: Since Ibuprofen is a known efflux pump inhibitor, our findings hint at the possible mechanism of Fluconazole resistance in most of our Candida isolates and suggest a potential therapeutic alternative that could be useful in the majority of Fluconazole-resistant clinical isolates of Candida.

Full Text

Duke Authors

Cited Authors

  • Sharma, M; Biswas, D; Kotwal, A; Thakuria, B; Kakati, B; Chauhan, BS; Patras, A

Published Date

  • January 2015

Published In

Volume / Issue

  • 9 / 1

Start / End Page

  • DC20 - DC22

PubMed ID

  • 25737988

Pubmed Central ID

  • 25737988

Electronic International Standard Serial Number (EISSN)

  • 0973-709X

International Standard Serial Number (ISSN)

  • 2249-782X

Digital Object Identifier (DOI)

  • 10.7860/JCDR/2015/10094.5494

Language

  • eng