A Farnesyltransferase Acts to Inhibit Ectopic Neurite Formation in C. elegans.
Journal Article
Genetic pathways that regulate nascent neurite formation play a critical role in neuronal morphogenesis. The core planar cell polarity components VANG-1/Van Gogh and PRKL-1/Prickle are involved in blocking inappropriate neurite formation in a subset of motor neurons in C. elegans. A genetic screen for mutants that display supernumerary neurites was performed to identify additional factors involved in this process. This screen identified mutations in fntb-1, the β subunit of farnesyltransferase. We show that fntb-1 is expressed in neurons and acts cell-autonomously to regulate neurite formation. Prickle proteins are known to be post-translationally modified by farnesylation at their C-terminal CAAX motifs. We show that PRKL-1 can be recruited to the plasma membrane in both a CAAX-dependent and CAAX-independent manner but that PRKL-1 can only inhibit neurite formation in a CAAX-dependent manner.
Full Text
Duke Authors
Cited Authors
- Carr, D; Sanchez-Alvarez, L; Imai, JH; Slatculescu, C; Noblett, N; Mao, L; Beese, L; Colavita, A
Published Date
- 2016
Published In
Volume / Issue
- 11 / 6
Start / End Page
- e0157537 -
PubMed ID
- 27300162
Pubmed Central ID
- 27300162
Electronic International Standard Serial Number (EISSN)
- 1932-6203
Digital Object Identifier (DOI)
- 10.1371/journal.pone.0157537
Language
- eng
Conference Location
- United States