A Farnesyltransferase Acts to Inhibit Ectopic Neurite Formation in C. elegans.

Published online

Journal Article

Genetic pathways that regulate nascent neurite formation play a critical role in neuronal morphogenesis. The core planar cell polarity components VANG-1/Van Gogh and PRKL-1/Prickle are involved in blocking inappropriate neurite formation in a subset of motor neurons in C. elegans. A genetic screen for mutants that display supernumerary neurites was performed to identify additional factors involved in this process. This screen identified mutations in fntb-1, the β subunit of farnesyltransferase. We show that fntb-1 is expressed in neurons and acts cell-autonomously to regulate neurite formation. Prickle proteins are known to be post-translationally modified by farnesylation at their C-terminal CAAX motifs. We show that PRKL-1 can be recruited to the plasma membrane in both a CAAX-dependent and CAAX-independent manner but that PRKL-1 can only inhibit neurite formation in a CAAX-dependent manner.

Full Text

Duke Authors

Cited Authors

  • Carr, D; Sanchez-Alvarez, L; Imai, JH; Slatculescu, C; Noblett, N; Mao, L; Beese, L; Colavita, A

Published Date

  • 2016

Published In

Volume / Issue

  • 11 / 6

Start / End Page

  • e0157537 -

PubMed ID

  • 27300162

Pubmed Central ID

  • 27300162

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0157537

Language

  • eng

Conference Location

  • United States