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Adenosine nucleotides in bile.

Publication ,  Journal Article
Chari, RS; Schutz, SM; Haebig, JE; Shimokura, GH; Cotton, PB; Fitz, JG; Meyers, WC
Published in: Am J Physiol
February 1996

Activation of purinergic receptors by ATP stimulates Cl- efflux in biliary epithelial cells. To determine whether purinergic agonists are present under physiological conditions, we have assayed mammalian bile for nucleotides and assessed whether hepatoma and cholangiocarcinoma cell lines are capable of nucleotide release. Bile samples were collected from human, rat, and pig donors and assayed for nucleotide concentrations by luminometry. ATP, ADP, and AMP were present in bile from each species, and the average total nucleotide concentration in human bile was 5.21 +/- 0.91 microM (n = 16). In an in vitro model of HTC rat hepatoma cells or Mz-ChA-1 cholangiocarcinoma cells on a superfused column, nucleotides were present in the effluent from each cell type. Addition of alpha, beta-methyleneadenosine 5'-diphosphate (50 microM) to inhibit 5'-nucleotidase activity increased AMP concentrations two- to threefold. Exposure to forskolin (100 microM) or ionomycin (2 microM) stimulated nucleotide release from cholangiocarcinoma but not hepatoma cells. These studies indicate that adenosine nucleotides are present in bile in concentrations sufficient to activate purinergic receptors. Purinergic receptor activation by local nucleotide release might constitute an autocrine and/or paracrine mechanism for modulation of biliary secretion.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

February 1996

Volume

270

Issue

2 Pt 1

Start / End Page

G246 / G252

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Swine
  • Rats, Inbred F344
  • Rats
  • Perfusion
  • Osmolar Concentration
  • Middle Aged
  • Male
  • Humans
  • Female
 

Citation

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Chari, R. S., Schutz, S. M., Haebig, J. E., Shimokura, G. H., Cotton, P. B., Fitz, J. G., & Meyers, W. C. (1996). Adenosine nucleotides in bile. Am J Physiol, 270(2 Pt 1), G246–G252. https://doi.org/10.1152/ajpgi.1996.270.2.G246
Chari, R. S., S. M. Schutz, J. E. Haebig, G. H. Shimokura, P. B. Cotton, J. G. Fitz, and W. C. Meyers. “Adenosine nucleotides in bile.Am J Physiol 270, no. 2 Pt 1 (February 1996): G246–52. https://doi.org/10.1152/ajpgi.1996.270.2.G246.
Chari RS, Schutz SM, Haebig JE, Shimokura GH, Cotton PB, Fitz JG, et al. Adenosine nucleotides in bile. Am J Physiol. 1996 Feb;270(2 Pt 1):G246–52.
Chari, R. S., et al. “Adenosine nucleotides in bile.Am J Physiol, vol. 270, no. 2 Pt 1, Feb. 1996, pp. G246–52. Pubmed, doi:10.1152/ajpgi.1996.270.2.G246.
Chari RS, Schutz SM, Haebig JE, Shimokura GH, Cotton PB, Fitz JG, Meyers WC. Adenosine nucleotides in bile. Am J Physiol. 1996 Feb;270(2 Pt 1):G246–G252.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

February 1996

Volume

270

Issue

2 Pt 1

Start / End Page

G246 / G252

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Swine
  • Rats, Inbred F344
  • Rats
  • Perfusion
  • Osmolar Concentration
  • Middle Aged
  • Male
  • Humans
  • Female