Genome-wide association study of sepsis in extremely premature infants.
Journal Article (Journal Article)
OBJECTIVE: To identify genetic variants associated with sepsis (early-onset and late-onset) using a genome-wide association (GWA) analysis in a cohort of extremely premature infants. STUDY DESIGN: Previously generated GWA data from the Neonatal Research Network's anonymised genomic database biorepository of extremely premature infants were used for this study. Sepsis was defined as culture-positive early-onset or late-onset sepsis or culture-proven meningitis. Genomic and whole-genome-amplified DNA was genotyped for 1.2 million single-nucleotide polymorphisms (SNPs); 91% of SNPs were successfully genotyped. We imputed 7.2 million additional SNPs. p Values and false discovery rates (FDRs) were calculated from multivariate logistic regression analysis adjusting for gender, gestational age and ancestry. Target statistical value was p<10-5. Secondary analyses assessed associations of SNPs with pathogen type. Pathway analyses were also run on primary and secondary end points. RESULTS: Data from 757 extremely premature infants were included: 351 infants with sepsis and 406 infants without sepsis. No SNPs reached genome-wide significance levels (5×10-8); two SNPs in proximity to FOXC2 and FOXL1 genes achieved target levels of significance. In secondary analyses, SNPs for ELMO1, IRAK2 (Gram-positive sepsis), RALA, IMMP2L (Gram-negative sepsis) and PIEZO2 (fungal sepsis) met target significance levels. Pathways associated with sepsis and Gram-negative sepsis included gap junctions, fibroblast growth factor receptors, regulators of cell division and interleukin-1-associated receptor kinase 2 (p values<0.001 and FDR<20%). CONCLUSIONS: No SNPs met genome-wide significance in this cohort of extremely low birthweight infants; however, areas of potential association and pathways meriting further study were identified.
Full Text
Duke Authors
Cited Authors
- Srinivasan, L; Page, G; Kirpalani, H; Murray, JC; Das, A; Higgins, RD; Carlo, WA; Bell, EF; Goldberg, RN; Schibler, K; Sood, BG; Stevenson, DK; Stoll, BJ; Van Meurs, KP; Johnson, KJ; Levy, J; McDonald, SA; Zaterka-Baxter, KM; Kennedy, KA; Sánchez, PJ; Duara, S; Walsh, MC; Shankaran, S; Wynn, JL; Cotten, CM; Eunice Kennedy Shriver National Institute of Child Health and Human Development Neonatal Research Network,
Published Date
- September 2017
Published In
Volume / Issue
- 102 / 5
Start / End Page
- F439 - F445
PubMed ID
- 28283553
Pubmed Central ID
- PMC5563277
Electronic International Standard Serial Number (EISSN)
- 1468-2052
Digital Object Identifier (DOI)
- 10.1136/archdischild-2016-311545
Language
- eng
Conference Location
- England