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Chondroitin Sulfate Glycosaminoglycan Matrices Promote Neural Stem Cell Maintenance and Neuroprotection Post-Traumatic Brain Injury.

Publication ,  Journal Article
Betancur, MI; Mason, HD; Alvarado-Velez, M; Holmes, PV; Bellamkonda, RV; Karumbaiah, L
Published in: ACS biomaterials science & engineering
March 2017

There are currently no effective treatments for moderate-to-severe traumatic brain injuries (TBIs). The paracrine functions of undifferentiated neural stem cells (NSCs) are believed to play a significant role in stimulating the repair and regeneration of injured brain tissue. We therefore hypothesized that fibroblast growth factor (FGF2) enriching chondroitin sulfate glycosaminoglycan (CS-GAG) matrices can maintain the undifferentiated state of neural stem cells (NSCs) and facilitate brain tissue repair subacutely post-TBI. Rats subjected to a controlled cortical impactor (CCI) induced TBI were intraparenchymally injected with CS-GAG matrices alone or with CS-GAG matrices containing PKH26GL labeled allogeneic NSCs. Nissl staining of brain tissue 4 weeks post-TBI demonstrated the significantly enhanced (p < 0.05) tissue protection in CS-GAG treated animals when compared to TBI only control, and NSC only treated animals. CS-GAG-NSC treated animals demonstrated significantly enhanced (p < 0.05) FGF2 retention, and maintenance of PKH26GL labeled NSCs as indicated by enhanced Sox1+ and Ki67+ cell presence over other differentiated cell types. Lastly, all treatment groups and sham controls exhibited a significantly (p < 0.05) attenuated GFAP+ reactive astrocyte presence in the lesion site when compared to TBI only controls.

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Published In

ACS biomaterials science & engineering

DOI

EISSN

2373-9878

ISSN

2373-9878

Publication Date

March 2017

Volume

3

Issue

3

Start / End Page

420 / 430

Related Subject Headings

  • 4003 Biomedical engineering
  • 0903 Biomedical Engineering
 

Citation

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ICMJE
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Betancur, M. I., Mason, H. D., Alvarado-Velez, M., Holmes, P. V., Bellamkonda, R. V., & Karumbaiah, L. (2017). Chondroitin Sulfate Glycosaminoglycan Matrices Promote Neural Stem Cell Maintenance and Neuroprotection Post-Traumatic Brain Injury. ACS Biomaterials Science & Engineering, 3(3), 420–430. https://doi.org/10.1021/acsbiomaterials.6b00805
Betancur, Martha I., Hannah D. Mason, Melissa Alvarado-Velez, Phillip V. Holmes, Ravi V. Bellamkonda, and Lohitash Karumbaiah. “Chondroitin Sulfate Glycosaminoglycan Matrices Promote Neural Stem Cell Maintenance and Neuroprotection Post-Traumatic Brain Injury.ACS Biomaterials Science & Engineering 3, no. 3 (March 2017): 420–30. https://doi.org/10.1021/acsbiomaterials.6b00805.
Betancur MI, Mason HD, Alvarado-Velez M, Holmes PV, Bellamkonda RV, Karumbaiah L. Chondroitin Sulfate Glycosaminoglycan Matrices Promote Neural Stem Cell Maintenance and Neuroprotection Post-Traumatic Brain Injury. ACS biomaterials science & engineering. 2017 Mar;3(3):420–30.
Betancur, Martha I., et al. “Chondroitin Sulfate Glycosaminoglycan Matrices Promote Neural Stem Cell Maintenance and Neuroprotection Post-Traumatic Brain Injury.ACS Biomaterials Science & Engineering, vol. 3, no. 3, Mar. 2017, pp. 420–30. Epmc, doi:10.1021/acsbiomaterials.6b00805.
Betancur MI, Mason HD, Alvarado-Velez M, Holmes PV, Bellamkonda RV, Karumbaiah L. Chondroitin Sulfate Glycosaminoglycan Matrices Promote Neural Stem Cell Maintenance and Neuroprotection Post-Traumatic Brain Injury. ACS biomaterials science & engineering. 2017 Mar;3(3):420–430.
Journal cover image

Published In

ACS biomaterials science & engineering

DOI

EISSN

2373-9878

ISSN

2373-9878

Publication Date

March 2017

Volume

3

Issue

3

Start / End Page

420 / 430

Related Subject Headings

  • 4003 Biomedical engineering
  • 0903 Biomedical Engineering