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Human Adipose-Derived Stem Cells Labeled with Plasmonic Gold Nanostars for Cellular Tracking and Photothermal Cancer Cell Ablation.

Publication ,  Journal Article
Shammas, RL; Fales, AM; Crawford, BM; Wisdom, AJ; Devi, GR; Brown, DA; Vo-Dinh, T; Hollenbeck, ST
Published in: Plast Reconstr Surg
April 2017

BACKGROUND: Gold nanostars are unique nanoplatforms that can be imaged in real time and transform light energy into heat to ablate cells. Adipose-derived stem cells migrate toward tumor niches in response to chemokines. The ability of adipose-derived stem cells to migrate and integrate into tumors makes them ideal vehicles for the targeted delivery of cancer nanotherapeutics. METHODS: To test the labeling efficiency of gold nanostars, undifferentiated adipose-derived stem cells were incubated with gold nanostars and a commercially available nanoparticle (Qtracker), then imaged using two-photon photoluminescence microscopy. The effects of gold nanostars on cell phenotype, proliferation, and viability were assessed with flow cytometry, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide metabolic assay, and trypan blue, respectively. Trilineage differentiation of gold nanostar-labeled adipose-derived stem cells was induced with the appropriate media. Photothermolysis was performed on adipose-derived stem cells cultured alone or in co-culture with SKBR3 cancer cells. RESULTS: Efficient uptake of gold nanostars occurred in adipose-derived stem cells, with persistence of the luminescent signal over 4 days. Labeling efficiency and signal quality were greater than with Qtracker. Gold nanostars did not affect cell phenotype, viability, or proliferation, and exhibited stronger luminescence than Qtracker throughout differentiation. Zones of complete ablation surrounding the gold nanostar-labeled adipose-derived stem cells were observed following photothermolysis in both monoculture and co-culture models. CONCLUSIONS: Gold nanostars effectively label adipose-derived stem cells without altering cell phenotype. Once labeled, photoactivation of gold nanostar-labeled adipose-derived stem cells ablates neighboring cancer cells, demonstrating the potential of adipose-derived stem cells as a vehicle for the delivery of site-specific cancer therapy.

Duke Scholars

Published In

Plast Reconstr Surg

DOI

EISSN

1529-4242

Publication Date

April 2017

Volume

139

Issue

4

Start / End Page

900e / 910e

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Surgery
  • Stem Cells
  • Staining and Labeling
  • Nanostructures
  • Humans
  • Gold
  • Cells, Cultured
  • Cell Tracking
  • Cell Differentiation
 

Citation

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Shammas, R. L., Fales, A. M., Crawford, B. M., Wisdom, A. J., Devi, G. R., Brown, D. A., … Hollenbeck, S. T. (2017). Human Adipose-Derived Stem Cells Labeled with Plasmonic Gold Nanostars for Cellular Tracking and Photothermal Cancer Cell Ablation. Plast Reconstr Surg, 139(4), 900e-910e. https://doi.org/10.1097/PRS.0000000000003187
Shammas, Ronnie L., Andrew M. Fales, Bridget M. Crawford, Amy J. Wisdom, Gayathri R. Devi, David A. Brown, Tuan Vo-Dinh, and Scott T. Hollenbeck. “Human Adipose-Derived Stem Cells Labeled with Plasmonic Gold Nanostars for Cellular Tracking and Photothermal Cancer Cell Ablation.Plast Reconstr Surg 139, no. 4 (April 2017): 900e-910e. https://doi.org/10.1097/PRS.0000000000003187.
Shammas RL, Fales AM, Crawford BM, Wisdom AJ, Devi GR, Brown DA, et al. Human Adipose-Derived Stem Cells Labeled with Plasmonic Gold Nanostars for Cellular Tracking and Photothermal Cancer Cell Ablation. Plast Reconstr Surg. 2017 Apr;139(4):900e-910e.
Shammas, Ronnie L., et al. “Human Adipose-Derived Stem Cells Labeled with Plasmonic Gold Nanostars for Cellular Tracking and Photothermal Cancer Cell Ablation.Plast Reconstr Surg, vol. 139, no. 4, Apr. 2017, pp. 900e-910e. Pubmed, doi:10.1097/PRS.0000000000003187.
Shammas RL, Fales AM, Crawford BM, Wisdom AJ, Devi GR, Brown DA, Vo-Dinh T, Hollenbeck ST. Human Adipose-Derived Stem Cells Labeled with Plasmonic Gold Nanostars for Cellular Tracking and Photothermal Cancer Cell Ablation. Plast Reconstr Surg. 2017 Apr;139(4):900e-910e.

Published In

Plast Reconstr Surg

DOI

EISSN

1529-4242

Publication Date

April 2017

Volume

139

Issue

4

Start / End Page

900e / 910e

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Surgery
  • Stem Cells
  • Staining and Labeling
  • Nanostructures
  • Humans
  • Gold
  • Cells, Cultured
  • Cell Tracking
  • Cell Differentiation