HIV-1 Consensus Envelope-Induced Broadly Binding Antibodies.
Antibodies that cross-react with multiple HIV-1 envelopes (Envs) are useful reagents for characterizing Env proteins and for soluble Env capture and purification assays. We previously reported 10 murine monoclonal antibodies induced by group M consensus Env, CON-6 immunization. Each demonstrated broad cross-reactivity to recombinant Envs. Here we characterized the Env epitopes to which they bind. Seven mapped to linear epitopes in gp120, five at the Env N-terminus, and two at the Env C-terminus. One antibody, 13D7, bound at the gp120 N-terminus (aa 30-42), reacted with HIV-1-infected CD4+ T cells, and when expressed in a human IgG1 backbone, mediated antibody-dependent cellular cytotoxicity. Antibody 18F11 bound at the gp120 C-terminus (aa 445-459) and reactivity was glycan dependent. Antibodies 13D7, 3B3, and 16H3 bound to 100 percent of HIV-1 Envs tested in ELISA and sodium dodecyl sulfate/polyacrylamide gel electrophoresis/western blot analysis. These data define the epitopes of monoclonal antibody reagents for characterization of recombinant Envs, one epitope of which is also expressed on the surface of HIV-1-infected CD4+ T cells.
Alam, S. Munir
Haynes, Barton Ford
Montefiori, David Charles
Tomaras, Georgia Doris
Wiehe, Kevin J
Meyerhoff, RR; Scearce, RM; Ogburn, DF; Lockwood, B; Pickeral, J; Kuraoka, M; Anasti, K; Eudailey, J; Eaton, A; Cooper, M; Wiehe, K; Montefiori, DC; Tomaras, G; Ferrari, G; Alam, SM; Liao, H-X; Korber, B; Gao, F; Haynes, BF
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