Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration.

Published online

Journal Article

Arf4 is proposed to be a critical regulator of membrane protein trafficking in early secretory pathway. More recently, Arf4 was also implicated in regulating ciliary trafficking, however, this has not been comprehensively tested in vivo. To directly address Arf4's role in ciliary transport, we deleted Arf4 specifically in either rod photoreceptor cells, kidney, or globally during the early postnatal period. Arf4 deletion in photoreceptors did not cause protein mislocalization or retinal degeneration, as expected if Arf4 played a role in protein transport to the ciliary outer segment. Likewise, Arf4 deletion in kidney did not cause cystic disease, as expected if Arf4 were involved in general ciliary trafficking. In contrast, global Arf4 deletion in the early postnatal period resulted in growth restriction, severe pancreatic degeneration and early death. These findings are consistent with Arf4 playing a critical role in endomembrane trafficking, particularly in the pancreas, but not in ciliary function.

Full Text

Duke Authors

Cited Authors

  • Pearring, JN; San Agustin, JT; Lobanova, ES; Gabriel, CJ; Lieu, EC; Monis, WJ; Stuck, MW; Strittmatter, L; Jaber, SM; Arshavsky, VY; Pazour, GJ

Published Date

  • April 2017

Published In

Volume / Issue

  • 13 / 4

Start / End Page

  • e1006740 -

PubMed ID

  • 28410364

Pubmed Central ID

  • 28410364

Electronic International Standard Serial Number (EISSN)

  • 1553-7404

Digital Object Identifier (DOI)

  • 10.1371/journal.pgen.1006740

Language

  • eng

Conference Location

  • United States