Fibroblastic niches prime T cell alloimmunity through Delta-like Notch ligands.

Journal Article

Alloimmune T cell responses induce graft-versus-host disease (GVHD), a serious complication of allogeneic bone marrow transplantation (allo-BMT). Although Notch signaling mediated by Delta-like 1/4 (DLL1/4) Notch ligands has emerged as a major regulator of GVHD pathogenesis, little is known about the timing of essential Notch signals and the cellular source of Notch ligands after allo-BMT. Here, we have shown that critical DLL1/4-mediated Notch signals are delivered to donor T cells during a short 48-hour window after transplantation in a mouse allo-BMT model. Stromal, but not hematopoietic, cells were the essential source of Notch ligands during in vivo priming of alloreactive T cells. GVHD could be prevented by selective inactivation of Dll1 and Dll4 in subsets of fibroblastic stromal cells that were derived from chemokine Ccl19-expressing host cells, including fibroblastic reticular cells and follicular dendritic cells. However, neither T cell recruitment into secondary lymphoid organs nor initial T cell activation was affected by Dll1/4 loss. Thus, we have uncovered a pathogenic function for fibroblastic stromal cells in alloimmune reactivity that can be dissociated from their homeostatic functions. Our results reveal what we believe to be a previously unrecognized Notch-mediated immunopathogenic role for stromal cell niches in secondary lymphoid organs after allo-BMT and define a framework of early cellular and molecular interactions that regulate T cell alloimmunity.

Full Text

Duke Authors

Cited Authors

  • Chung, J; Ebens, CL; Perkey, E; Radojcic, V; Koch, U; Scarpellino, L; Tong, A; Allen, F; Wood, S; Feng, J; Friedman, A; Granadier, D; Tran, IT; Chai, Q; Onder, L; Yan, M; Reddy, P; Blazar, BR; Huang, AY; Brennan, TV; Bishop, DK; Ludewig, B; Siebel, CW; Radtke, F; Luther, SA; Maillard, I

Published Date

  • April 2017

Published In

Volume / Issue

  • 127 / 4

Start / End Page

  • 1574 - 1588

PubMed ID

  • 28319044

Electronic International Standard Serial Number (EISSN)

  • 1558-8238

International Standard Serial Number (ISSN)

  • 0021-9738

Digital Object Identifier (DOI)

  • 10.1172/jci89535

Language

  • eng