Introducing MX Cassettes into Saccharomyces cerevisiae.
Publication
, Journal Article
McCusker, JH
Published in: Cold Spring Harb Protoc
April 3, 2017
The Saccharomyces cerevisiae genome can be readily and precisely modified with the use of knock out (KO) marker cassettes to delete genes. The most frequently used family of KO cassettes is the MX cassettes. This protocol describes how to use the different types of MX cassettes by selecting for prototrophy, utilization of cytosine or acetamide as a sole nitrogen source, or resistance to one of six different drugs.
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Published In
Cold Spring Harb Protoc
DOI
EISSN
1559-6095
Publication Date
April 3, 2017
Volume
2017
Issue
4
Start / End Page
pdb.prot088104
Location
United States
Related Subject Headings
- Selection, Genetic
- Saccharomyces cerevisiae
- Recombination, Genetic
- Mutagenesis, Insertional
- Microbiological Techniques
- Gene Knockout Techniques
- 3101 Biochemistry and cell biology
- 1103 Clinical Sciences
- 0601 Biochemistry and Cell Biology
- 0306 Physical Chemistry (incl. Structural)
Citation
APA
Chicago
ICMJE
MLA
NLM
McCusker, J. H. (2017). Introducing MX Cassettes into Saccharomyces cerevisiae. Cold Spring Harb Protoc, 2017(4), pdb.prot088104. https://doi.org/10.1101/pdb.prot088104
McCusker, John H. “Introducing MX Cassettes into Saccharomyces cerevisiae.” Cold Spring Harb Protoc 2017, no. 4 (April 3, 2017): pdb.prot088104. https://doi.org/10.1101/pdb.prot088104.
McCusker JH. Introducing MX Cassettes into Saccharomyces cerevisiae. Cold Spring Harb Protoc. 2017 Apr 3;2017(4):pdb.prot088104.
McCusker, John H. “Introducing MX Cassettes into Saccharomyces cerevisiae.” Cold Spring Harb Protoc, vol. 2017, no. 4, Apr. 2017, p. pdb.prot088104. Pubmed, doi:10.1101/pdb.prot088104.
McCusker JH. Introducing MX Cassettes into Saccharomyces cerevisiae. Cold Spring Harb Protoc. 2017 Apr 3;2017(4):pdb.prot088104.
Published In
Cold Spring Harb Protoc
DOI
EISSN
1559-6095
Publication Date
April 3, 2017
Volume
2017
Issue
4
Start / End Page
pdb.prot088104
Location
United States
Related Subject Headings
- Selection, Genetic
- Saccharomyces cerevisiae
- Recombination, Genetic
- Mutagenesis, Insertional
- Microbiological Techniques
- Gene Knockout Techniques
- 3101 Biochemistry and cell biology
- 1103 Clinical Sciences
- 0601 Biochemistry and Cell Biology
- 0306 Physical Chemistry (incl. Structural)