Introducing MX Cassettes into Saccharomyces cerevisiae.
The Saccharomyces cerevisiae genome can be readily and precisely modified with the use of knock out (KO) marker cassettes to delete genes. The most frequently used family of KO cassettes is the MX cassettes. This protocol describes how to use the different types of MX cassettes by selecting for prototrophy, utilization of cytosine or acetamide as a sole nitrogen source, or resistance to one of six different drugs.
Volume / Issue
Start / End Page
Electronic International Standard Serial Number (EISSN)
Digital Object Identifier (DOI)