Introducing MX Cassettes into Saccharomyces cerevisiae.

Journal Article (Journal Article)

The Saccharomyces cerevisiae genome can be readily and precisely modified with the use of knock out (KO) marker cassettes to delete genes. The most frequently used family of KO cassettes is the MX cassettes. This protocol describes how to use the different types of MX cassettes by selecting for prototrophy, utilization of cytosine or acetamide as a sole nitrogen source, or resistance to one of six different drugs.

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Duke Authors

McCusker, John Henry

Cited Authors

McCusker, JH

Published Date

April 3, 2017

Published In

Cold Spring Harbor Protocols

Volume / Issue

2017 / 4

Start / End Page

pdb.prot088104 -

PubMed ID

28373487

Electronic International Standard Serial Number (EISSN)

1559-6095

Digital Object Identifier (DOI)

10.1101/pdb.prot088104

Language

Conference Location

United States

Scholars@Duke