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Introducing MX Cassettes into Saccharomyces cerevisiae.

Publication ,  Journal Article
McCusker, JH
Published in: Cold Spring Harb Protoc
April 3, 2017

The Saccharomyces cerevisiae genome can be readily and precisely modified with the use of knock out (KO) marker cassettes to delete genes. The most frequently used family of KO cassettes is the MX cassettes. This protocol describes how to use the different types of MX cassettes by selecting for prototrophy, utilization of cytosine or acetamide as a sole nitrogen source, or resistance to one of six different drugs.

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Published In

Cold Spring Harb Protoc

DOI

EISSN

1559-6095

Publication Date

April 3, 2017

Volume

2017

Issue

4

Start / End Page

pdb.prot088104

Location

United States

Related Subject Headings

  • Selection, Genetic
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Mutagenesis, Insertional
  • Microbiological Techniques
  • Gene Knockout Techniques
  • 3101 Biochemistry and cell biology
  • 1103 Clinical Sciences
  • 0601 Biochemistry and Cell Biology
  • 0306 Physical Chemistry (incl. Structural)
 

Citation

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McCusker, J. H. (2017). Introducing MX Cassettes into Saccharomyces cerevisiae. Cold Spring Harb Protoc, 2017(4), pdb.prot088104. https://doi.org/10.1101/pdb.prot088104
McCusker, John H. “Introducing MX Cassettes into Saccharomyces cerevisiae.Cold Spring Harb Protoc 2017, no. 4 (April 3, 2017): pdb.prot088104. https://doi.org/10.1101/pdb.prot088104.
McCusker JH. Introducing MX Cassettes into Saccharomyces cerevisiae. Cold Spring Harb Protoc. 2017 Apr 3;2017(4):pdb.prot088104.
McCusker, John H. “Introducing MX Cassettes into Saccharomyces cerevisiae.Cold Spring Harb Protoc, vol. 2017, no. 4, Apr. 2017, p. pdb.prot088104. Pubmed, doi:10.1101/pdb.prot088104.
McCusker JH. Introducing MX Cassettes into Saccharomyces cerevisiae. Cold Spring Harb Protoc. 2017 Apr 3;2017(4):pdb.prot088104.

Published In

Cold Spring Harb Protoc

DOI

EISSN

1559-6095

Publication Date

April 3, 2017

Volume

2017

Issue

4

Start / End Page

pdb.prot088104

Location

United States

Related Subject Headings

  • Selection, Genetic
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Mutagenesis, Insertional
  • Microbiological Techniques
  • Gene Knockout Techniques
  • 3101 Biochemistry and cell biology
  • 1103 Clinical Sciences
  • 0601 Biochemistry and Cell Biology
  • 0306 Physical Chemistry (incl. Structural)