Tissue response to particulate polymethylmethacrylate in mice with various immune deficiencies.

Journal Article (Journal Article)

We examined the tissue response to subcutaneous injections of particulate polymethylmethacrylate powder in fully immunocompetent C3Hf/Sed mice as well as three strains of mice with different levels of lymphocyte dysfunction. Five weeks after the injection, we found clearly demarcated granulomas. Histological and immunohistochemical studies showed that these granulomas were similar among all strains, with either paucity or absence of lymphoid cells. In situ hybridization with use of complementary RNA probes indicated that macrophages were synthesizing interleukin-1 beta messenger RNA (mRNA), a marker of macrophage activation, and a cytokine implicated in pathological bone resorption. We concluded that, in mice, there is a lymphocyte-independent pathway of macrophage activation in response to particulate polymethylmethacrylate. This suggests that the foreign-body response to particulate orthopaedic biomaterials is macrophage-initiated and maintained and that lymphocytes are not essential to this response, although they may modulate it.

Full Text

Duke Authors

Cited Authors

  • Jiranek, W; Jasty, M; Wang, JT; Bragdon, C; Wolfe, H; Goldberg, M; Harris, W

Published Date

  • November 1995

Published In

Volume / Issue

  • 77 / 11

Start / End Page

  • 1650 - 1661

PubMed ID

  • 7593074

International Standard Serial Number (ISSN)

  • 0021-9355

Digital Object Identifier (DOI)

  • 10.2106/00004623-199511000-00003


  • eng

Conference Location

  • United States