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Transforming growth factor-beta 1 and 2 in the synovial-like interface membrane between implant and bone in loosening of total hip arthroplasty.

Publication ,  Journal Article
Konttinen, YT; Waris, V; Xu, JW; Jiranek, WA; Sorsa, T; Virtanen, I; Santavirta, S
Published in: J Rheumatol
April 1997

OBJECTIVE: Development of a synovial-like membrane in the implant-bone or cement-bone interface has been linked to aseptic loosening of total hip arthroplasties (THA). This tissue consists of a fibrous stroma containing blood vessels and macrophages, but with relatively few lymphocytes, compared to "autoimmune" rheumatoid synovitis. Our aim was to examine transforming growth factor-beta (TGF-beta) in the synovial-like membrane of the interface and pseudocapsular tissue of loose THA and compare it to control knee synovial membrane. METHODS: Twenty samples obtained from 10 patients with loose THA at revisions performed for aseptic loosening and 10 samples of knee synovial membrane as controls were analyzed for TGF-beta expression using rabbit antihuman TGF-beta 1 and TGF-beta 2 IgG in immunohistochemical staining. Results were quantitated by a semi-automatic VIDAS image analysis system. RESULTS: Immunoperoxidase staining disclosed TGF-beta in macrophages and fibroblasts and also in some vascular endothelial cells and in occasional lymphocytes. Image analysis showed an increased number of positive cells/mm2 of both TGF-beta 1 (2327 +/- 212 vs 946 +/- 136; p < 0.01) and TGF-beta 2 (2292 +/- 594 vs 311 +/- 113; p < 0.01) compared to the control tissue. Increased expression of both TGF-beta 1 and TGF-beta 2 was also shown in the pseudocapsule (3210 +/- 585 and 1796 +/- 214). Use of cement or type of alloy did not seem to have any great effect on local expression of TGF-beta. CONCLUSION: Profibrotic and immunosuppressive TGF-beta are increased in the synovial-like membrane in periprosthetic tissues around loose hip prostheses. They may play a role in the formation, maintenance, and growth of the interface tissue, and thus in the aseptic loosening of THA.

Duke Scholars

Published In

J Rheumatol

ISSN

0315-162X

Publication Date

April 1997

Volume

24

Issue

4

Start / End Page

694 / 701

Location

Canada

Related Subject Headings

  • Transforming Growth Factor beta
  • Synovial Membrane
  • Middle Aged
  • Membranes
  • Male
  • Immunohistochemistry
  • Humans
  • Hip Prosthesis
  • Female
  • Arthritis & Rheumatology
 

Citation

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MLA
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Konttinen, Y. T., Waris, V., Xu, J. W., Jiranek, W. A., Sorsa, T., Virtanen, I., & Santavirta, S. (1997). Transforming growth factor-beta 1 and 2 in the synovial-like interface membrane between implant and bone in loosening of total hip arthroplasty. J Rheumatol, 24(4), 694–701.
Konttinen, Y. T., V. Waris, J. W. Xu, W. A. Jiranek, T. Sorsa, I. Virtanen, and S. Santavirta. “Transforming growth factor-beta 1 and 2 in the synovial-like interface membrane between implant and bone in loosening of total hip arthroplasty.J Rheumatol 24, no. 4 (April 1997): 694–701.
Konttinen YT, Waris V, Xu JW, Jiranek WA, Sorsa T, Virtanen I, et al. Transforming growth factor-beta 1 and 2 in the synovial-like interface membrane between implant and bone in loosening of total hip arthroplasty. J Rheumatol. 1997 Apr;24(4):694–701.
Konttinen YT, Waris V, Xu JW, Jiranek WA, Sorsa T, Virtanen I, Santavirta S. Transforming growth factor-beta 1 and 2 in the synovial-like interface membrane between implant and bone in loosening of total hip arthroplasty. J Rheumatol. 1997 Apr;24(4):694–701.

Published In

J Rheumatol

ISSN

0315-162X

Publication Date

April 1997

Volume

24

Issue

4

Start / End Page

694 / 701

Location

Canada

Related Subject Headings

  • Transforming Growth Factor beta
  • Synovial Membrane
  • Middle Aged
  • Membranes
  • Male
  • Immunohistochemistry
  • Humans
  • Hip Prosthesis
  • Female
  • Arthritis & Rheumatology