Tissue-Specific Transcriptome Profiling in Arabidopsis Roots.
Journal Article (Journal Article)
Spatiotemporal transcriptome profiles from specific tissues are critical for understanding plant development and responses to the environment. One approach to isolate specific tissues is fluorescence-activated cell sorting (FACS). In this chapter, we outline methods for the FACS isolation of root protoplasts followed by transcriptome profiling using RNA sequencing.
Full Text
Duke Authors
Cited Authors
- Sparks, EE; Benfey, PN
Published Date
- January 2017
Published In
Volume / Issue
- 1610 /
Start / End Page
- 107 - 122
PubMed ID
- 28439860
Pubmed Central ID
- 28439860
Electronic International Standard Serial Number (EISSN)
- 1940-6029
International Standard Serial Number (ISSN)
- 1064-3745
Digital Object Identifier (DOI)
- 10.1007/978-1-4939-7003-2_8
Language
- eng