Tissue-Specific Transcriptome Profiling in Arabidopsis Roots.

Journal Article (Journal Article)

Spatiotemporal transcriptome profiles from specific tissues are critical for understanding plant development and responses to the environment. One approach to isolate specific tissues is fluorescence-activated cell sorting (FACS). In this chapter, we outline methods for the FACS isolation of root protoplasts followed by transcriptome profiling using RNA sequencing.

Full Text

Duke Authors

Cited Authors

  • Sparks, EE; Benfey, PN

Published Date

  • January 2017

Published In

Volume / Issue

  • 1610 /

Start / End Page

  • 107 - 122

PubMed ID

  • 28439860

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-7003-2_8


  • eng