Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ.

Published online

Journal Article

FtsZ is an essential protein for bacterial cell division, where it forms the cytoskeletal scaffold and may generate the constriction force. We have found previously that some mutant and foreign FtsZ that do not complement an ftsZ null can function for cell division in E. coli upon acquisition of a suppressor mutation somewhere in the genome. We have now identified, via whole genome re-sequencing, single nucleotide polymorphisms in 11 different suppressor strains. Most of the mutations are in genes of various metabolic pathways, which may modulate cell division indirectly. Mutations in three genes, ispA, accD and nlpI, may be more directly involved in cell division. In addition to the genomic suppressor mutations, we identified intragenic suppressors of three FtsZ point mutants (R174A, E250K and L272V).

Full Text

Duke Authors

Cited Authors

  • Gardner, KAJA; Osawa, M; Erickson, HP

Published Date

  • 2017

Published In

Volume / Issue

  • 12 / 4

Start / End Page

  • e0176643 -

PubMed ID

  • 28445510

Pubmed Central ID

  • 28445510

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0176643

Language

  • eng

Conference Location

  • United States