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The application of permanent middle cerebral artery ligation in the mouse.

Publication ,  Journal Article
Colak, G; Filiano, AJ; Johnson, GVW
Published in: J Vis Exp
July 25, 2011

Focal cerebral ischemia is among the most common type of stroke seen in patients. Due to the clinical significance there has been a prolonged effort to develop suitable animal models to study the events that unfold during ischemic insult. These techniques include transient or permanent, focal or global ischemia models using many different animal models, with the most common being rodents. The permanent MCA ligation method which is also referred as pMCAo in the literature is used extensively as a focal ischemia model in rodents. This method was originally described for rats by Tamura et al. in 1981. In this protocol a craniotomy was used to access the MCA and the proximal regions were occluded by electrocoagulation. The infarcts involve mostly cortical and sometimes striatal regions depending on the location of the occlusion. This technique is now well established and used in many laboratories. Early use of this technique led to the definition and description of "infarct core" and "penumbra", and it is often used to evaluate potential neuroprotective compounds. Although the initial studies were performed in rats, permanent MCA ligation has been used successfully in mice with slight modifications. This model yields reproducible infarcts and increased post-survival rates. Approximately 80% of the ischemic strokes in humans happen in the MCA area and thus this is a very relevant model for stroke studies. Currently, there is a paucity of effective treatments available to stroke patients, and thus there is a need for good models to test potential pharmacological compounds and evaluate physiological outcomes. This method can also be used for studying intracellular hypoxia response mechanisms in vivo. Here, we present the MCA ligation surgery in a C57/BL6 mouse. We describe the pre-surgical preparation, MCA ligation surgery and 2,3,5 Triphenyltetrazolium chloride (TTC) staining for quantification of infarct volumes.

Duke Scholars

Published In

J Vis Exp

DOI

EISSN

1940-087X

Publication Date

July 25, 2011

Issue

53

Location

United States

Related Subject Headings

  • Tetrazolium Salts
  • Staining and Labeling
  • Middle Cerebral Artery
  • Mice, Inbred C57BL
  • Mice
  • Ligation
  • Disease Models, Animal
  • Brain Ischemia
  • Animals
  • 3101 Biochemistry and cell biology
 

Citation

APA
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ICMJE
MLA
NLM
Colak, G., Filiano, A. J., & Johnson, G. V. W. (2011). The application of permanent middle cerebral artery ligation in the mouse. J Vis Exp, (53). https://doi.org/10.3791/3039
Colak, Gozde, Anthony J. Filiano, and Gail V. W. Johnson. “The application of permanent middle cerebral artery ligation in the mouse.J Vis Exp, no. 53 (July 25, 2011). https://doi.org/10.3791/3039.
Colak G, Filiano AJ, Johnson GVW. The application of permanent middle cerebral artery ligation in the mouse. J Vis Exp. 2011 Jul 25;(53).
Colak, Gozde, et al. “The application of permanent middle cerebral artery ligation in the mouse.J Vis Exp, no. 53, July 2011. Pubmed, doi:10.3791/3039.
Colak G, Filiano AJ, Johnson GVW. The application of permanent middle cerebral artery ligation in the mouse. J Vis Exp. 2011 Jul 25;(53).

Published In

J Vis Exp

DOI

EISSN

1940-087X

Publication Date

July 25, 2011

Issue

53

Location

United States

Related Subject Headings

  • Tetrazolium Salts
  • Staining and Labeling
  • Middle Cerebral Artery
  • Mice, Inbred C57BL
  • Mice
  • Ligation
  • Disease Models, Animal
  • Brain Ischemia
  • Animals
  • 3101 Biochemistry and cell biology