Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry.

Published

Journal Article

A variety of immune cell subsets contribute to the pathogenesis of hypertension and associated kidney damage following inappropriate activation of the renin-angiotensin system (RAS). These immune cell subsets often express common surface markers, which complicates their separation and characterization in vivo. Accordingly, flow cytometry has become an invaluable tool for parsing immune cell populations because this technique permits the simultaneous detection of up to 18 markers on a single cell. Below we describe a process by which one can determine the immune cell subsets in the kidney via flow cytometry.

Full Text

Duke Authors

Cited Authors

  • Rudemiller, NP; Crowley, SD

Published Date

  • 2017

Published In

Volume / Issue

  • 1614 /

Start / End Page

  • 87 - 98

PubMed ID

  • 28500598

Pubmed Central ID

  • 28500598

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-7030-8_8

Language

  • eng

Conference Location

  • United States