Transcriptional profile of hippocampal dentate granule cells in four rat epilepsy models.

Journal Article (Journal Article)

Global expression profiling of neurologic or psychiatric disorders has been confounded by variability among laboratories, animal models, tissues sampled, and experimental platforms, with the result being that few genes demonstrate consistent expression changes. We attempted to minimize these confounds by pooling dentate granule cell transcriptional profiles from 164 rats in seven laboratories, using three status epilepticus (SE) epilepsy models (pilocarpine, kainate, self-sustained SE), plus amygdala kindling. In each epilepsy model, RNA was harvested from laser-captured dentate granule cells from six rats at four time points early in the process of developing epilepsy, and data were collected from two independent laboratories in each rodent model except SSSE. Hierarchical clustering of differentially-expressed transcripts in the three SE models revealed complete separation between controls and SE rats isolated 1 day after SE. However, concordance of gene expression changes in the SE models was only 26-38% between laboratories, and 4.5% among models, validating the consortium approach. Transcripts with unusually highly variable control expression across laboratories provide a 'red herring' list for low-powered studies.

Full Text

Duke Authors

Cited Authors

  • Dingledine, R; Coulter, DA; Fritsch, B; Gorter, JA; Lelutiu, N; McNamara, J; Nadler, JV; Pitkänen, A; Rogawski, MA; Skene, P; Sloviter, RS; Wang, Y; Wadman, WJ; Wasterlain, C; Roopra, A

Published Date

  • May 9, 2017

Published In

Volume / Issue

  • 4 /

Start / End Page

  • 170061 -

PubMed ID

  • 28485718

Pubmed Central ID

  • PMC5423390

Electronic International Standard Serial Number (EISSN)

  • 2052-4463

Digital Object Identifier (DOI)

  • 10.1038/sdata.2017.61


  • eng

Conference Location

  • England