Structured illumination multimodal 3D-resolved quantitative phase and fluorescence sub-diffraction microscopy.
Published
Journal Article
Sub-diffraction resolution imaging has played a pivotal role in biological research by visualizing key, but previously unresolvable, sub-cellular structures. Unfortunately, applications of far-field sub-diffraction resolution are currently divided between fluorescent and coherent-diffraction regimes, and a multimodal sub-diffraction technique that bridges this gap has not yet been demonstrated. Here we report that structured illumination (SI) allows multimodal sub-diffraction imaging of both coherent quantitative-phase (QP) and fluorescence. Due to SI's conventionally fluorescent applications, we first demonstrate the principle of SI-enabled three-dimensional (3D) QP sub-diffraction imaging with calibration microspheres. Image analysis confirmed enhanced lateral and axial resolutions over diffraction-limited QP imaging, and established striking parallels between coherent SI and conventional optical diffraction tomography. We next introduce an optical system utilizing SI to achieve 3D sub-diffraction, multimodal QP/fluorescent visualization of A549 biological cells fluorescently tagged for F-actin. Our results suggest that SI has a unique utility in studying biological phenomena with significant molecular, biophysical, and biochemical components.
Full Text
Duke Authors
Cited Authors
- Chowdhury, S; Eldridge, WJ; Wax, A; Izatt, JA
Published Date
- May 2017
Published In
Volume / Issue
- 8 / 5
Start / End Page
- 2496 - 2518
PubMed ID
- 28663887
Pubmed Central ID
- 28663887
Electronic International Standard Serial Number (EISSN)
- 2156-7085
International Standard Serial Number (ISSN)
- 2156-7085
Digital Object Identifier (DOI)
- 10.1364/boe.8.002496
Language
- eng