Dmrt1 induces the male pathway in a turtle species with temperature-dependent sex determination.

Published

Journal Article

The molecular mechanism underlying temperature-dependent sex determination (TSD) has been a long-standing mystery; in particular, the thermosensitive genetic triggers for gonadal sex differentiation are largely unknown. Here, we have characterized a conserved DM domain gene, Dmrt1, in the red-eared slider turtle Trachemys scripta (T. scripta), which exhibits TSD. We found that Dmrt1 has a temperature-dependent, sexually dimorphic expression pattern, preceding gonadal sex differentiation, and is capable of responding rapidly to temperature shifts and aromatase inhibitor treatment. Most importantly, loss- and gain-of-function analyses provide solid evidence that Dmrt1 is both necessary and sufficient to initiate male development in T. scripta Furthermore, the DNA methylation dynamics of the Dmrt1 promoter are tightly correlated with temperature and could mediate the impact of temperature on sex determination. Collectively, our findings demonstrate that Dmrt1 is a candidate master male sex-determining gene in this TSD species, consistent with the idea that DM domain genes are conserved during the evolution of sex determination mechanisms.

Full Text

Duke Authors

Cited Authors

  • Ge, C; Ye, J; Zhang, H; Zhang, Y; Sun, W; Sang, Y; Capel, B; Qian, G

Published Date

  • June 15, 2017

Published In

Volume / Issue

  • 144 / 12

Start / End Page

  • 2222 - 2233

PubMed ID

  • 28506988

Pubmed Central ID

  • 28506988

Electronic International Standard Serial Number (EISSN)

  • 1477-9129

Digital Object Identifier (DOI)

  • 10.1242/dev.152033

Language

  • eng

Conference Location

  • England