Skip to main content
Journal cover image

uORF-mediated translation allows engineered plant disease resistance without fitness costs.

Publication ,  Journal Article
Xu, G; Yuan, M; Ai, C; Liu, L; Zhuang, E; Karapetyan, S; Wang, S; Dong, X
Published in: Nature
May 2017

Controlling plant disease has been a struggle for humankind since the advent of agriculture. Studies of plant immune mechanisms have led to strategies of engineering resistant crops through ectopic transcription of plants' own defence genes, such as the master immune regulatory gene NPR1 (ref. 1). However, enhanced resistance obtained through such strategies is often associated with substantial penalties to fitness, making the resulting products undesirable for agricultural applications. To remedy this problem, we sought more stringent mechanisms of expressing defence proteins. On the basis of our latest finding that translation of key immune regulators, such as TBF1 (ref. 3), is rapidly and transiently induced upon pathogen challenge (see accompanying paper), we developed a 'TBF1-cassette' consisting of not only the immune-inducible promoter but also two pathogen-responsive upstream open reading frames (uORFsTBF1) of the TBF1 gene. Here we demonstrate that inclusion of uORFsTBF1-mediated translational control over the production of snc1-1 (an autoactivated immune receptor) in Arabidopsis thaliana and AtNPR1 in rice enables us to engineer broad-spectrum disease resistance without compromising plant fitness in the laboratory or in the field. This broadly applicable strategy may lead to decreased pesticide use and reduce the selective pressure for resistant pathogens.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nature

DOI

EISSN

1476-4687

ISSN

0028-0836

Publication Date

May 2017

Volume

545

Issue

7655

Start / End Page

491 / 494

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Protein Biosynthesis
  • Promoter Regions, Genetic
  • Plant Proteins
  • Plant Immunity
  • Plant Diseases
  • Oryza
  • Open Reading Frames
  • Heat-Shock Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Xu, G., Yuan, M., Ai, C., Liu, L., Zhuang, E., Karapetyan, S., … Dong, X. (2017). uORF-mediated translation allows engineered plant disease resistance without fitness costs. Nature, 545(7655), 491–494. https://doi.org/10.1038/nature22372
Xu, Guoyong, Meng Yuan, Chaoren Ai, Lijing Liu, Edward Zhuang, Sargis Karapetyan, Shiping Wang, and Xinnian Dong. “uORF-mediated translation allows engineered plant disease resistance without fitness costs.Nature 545, no. 7655 (May 2017): 491–94. https://doi.org/10.1038/nature22372.
Xu G, Yuan M, Ai C, Liu L, Zhuang E, Karapetyan S, et al. uORF-mediated translation allows engineered plant disease resistance without fitness costs. Nature. 2017 May;545(7655):491–4.
Xu, Guoyong, et al. “uORF-mediated translation allows engineered plant disease resistance without fitness costs.Nature, vol. 545, no. 7655, May 2017, pp. 491–94. Epmc, doi:10.1038/nature22372.
Xu G, Yuan M, Ai C, Liu L, Zhuang E, Karapetyan S, Wang S, Dong X. uORF-mediated translation allows engineered plant disease resistance without fitness costs. Nature. 2017 May;545(7655):491–494.
Journal cover image

Published In

Nature

DOI

EISSN

1476-4687

ISSN

0028-0836

Publication Date

May 2017

Volume

545

Issue

7655

Start / End Page

491 / 494

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Protein Biosynthesis
  • Promoter Regions, Genetic
  • Plant Proteins
  • Plant Immunity
  • Plant Diseases
  • Oryza
  • Open Reading Frames
  • Heat-Shock Proteins