Peptide-Functionalized Fluorescent Particles for In Situ Detection of Nitric Oxide via Peroxynitrite-Mediated Nitration.

Published

Journal Article

Nitric oxide (NO) is a free radical signaling molecule that plays a crucial role in modulating physiological homeostasis across multiple biological systems. NO dysregulation is linked to the pathogenesis of multiple diseases; therefore, its quantification is important for understanding pathophysiological processes. The detection of NO is challenging, typically limited by its reactive nature and short half-life. Additionally, the presence of interfering analytes and accessibility to biological fluids in the native tissues make the measurement technically challenging and often unreliable. Here, a bio-inspired peptide-based NO sensor is developed, which detects NO-derived oxidants, predominately peroxynitrite-mediated nitration of tyrosine residues. It is demonstrated that these peptide-based NO sensors can detect peroxynitrite-mediated nitration in response to physiological shear stress by endothelial cells in vitro. Using the peptide-conjugated fluorescent particle immunoassay, peroxynitrite-mediated nitration activity with a detection limit of ≈100 × 10-9 m is detected. This study envisions that the NO detection platform can be applied to a multitude of applications including monitoring of NO activity in healthy and diseased tissues, localized detection of NO production of specific cells, and cell-based/therapeutic screening of peroxynitrite levels to monitor pronitroxidative stress in biological samples.

Full Text

Duke Authors

Cited Authors

  • Chang, JYH; Chow, LW; Dismuke, WM; Ethier, CR; Stevens, MM; Stamer, WD; Overby, DR

Published Date

  • August 2017

Published In

Volume / Issue

  • 6 / 16

PubMed ID

  • 28512791

Pubmed Central ID

  • 28512791

Electronic International Standard Serial Number (EISSN)

  • 2192-2659

International Standard Serial Number (ISSN)

  • 2192-2640

Digital Object Identifier (DOI)

  • 10.1002/adhm.201700383

Language

  • eng