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Mechanotransmission in endothelial cells subjected to oscillatory and multi-directional shear flow.

Publication ,  Journal Article
Dabagh, M; Jalali, P; Butler, PJ; Randles, A; Tarbell, JM
Published in: Journal of the Royal Society, Interface
May 2017

Local haemodynamics are linked to the non-uniform distribution of atherosclerosic lesions in arteries. Low and oscillatory (reversing in the axial flow direction) wall shear stress (WSS) induce inflammatory responses in endothelial cells (ECs) mediating disease localization. The objective of this study is to investigate computationally how the flow direction (reflected in WSS variation on the EC surface over time) influences the forces experienced by structural components of ECs that are believed to play important roles in mechanotransduction. A three-dimensional, multi-scale, multi-component, viscoelastic model of focally adhered ECs is developed, in which oscillatory WSS (reversing or non-reversing) parallel to the principal flow direction, or multi-directional oscillatory WSS with reversing axial and transverse components are applied over the EC surface. The computational model includes the glycocalyx layer, actin cortical layer, nucleus, cytoskeleton, focal adhesions (FAs), stress fibres and adherens junctions (ADJs). We show the distinct effects of atherogenic flow profiles (reversing unidirectional flow and reversing multi-directional flow) on subcellular structures relative to non-atherogenic flow (non-reversing flow). Reversing flow lowers stresses and strains due to viscoelastic effects, and multi-directional flow alters stress on the ADJs perpendicular to the axial flow direction. The simulations predict forces on integrins, ADJ filaments and other substructures in the range that activate mechanotransduction.

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Published In

Journal of the Royal Society, Interface

DOI

EISSN

1742-5662

ISSN

1742-5689

Publication Date

May 2017

Volume

14

Issue

130

Start / End Page

20170185

Related Subject Headings

  • Stress, Mechanical
  • Shear Strength
  • Models, Biological
  • General Science & Technology
  • Endothelial Cells
  • Computer Simulation
  • Cell Communication
  • Cell Adhesion
  • Biomechanical Phenomena
  • Adherens Junctions
 

Citation

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Dabagh, M., Jalali, P., Butler, P. J., Randles, A., & Tarbell, J. M. (2017). Mechanotransmission in endothelial cells subjected to oscillatory and multi-directional shear flow. Journal of the Royal Society, Interface, 14(130), 20170185. https://doi.org/10.1098/rsif.2017.0185
Dabagh, Mahsa, Payman Jalali, Peter J. Butler, Amanda Randles, and John M. Tarbell. “Mechanotransmission in endothelial cells subjected to oscillatory and multi-directional shear flow.Journal of the Royal Society, Interface 14, no. 130 (May 2017): 20170185. https://doi.org/10.1098/rsif.2017.0185.
Dabagh M, Jalali P, Butler PJ, Randles A, Tarbell JM. Mechanotransmission in endothelial cells subjected to oscillatory and multi-directional shear flow. Journal of the Royal Society, Interface. 2017 May;14(130):20170185.
Dabagh, Mahsa, et al. “Mechanotransmission in endothelial cells subjected to oscillatory and multi-directional shear flow.Journal of the Royal Society, Interface, vol. 14, no. 130, May 2017, p. 20170185. Epmc, doi:10.1098/rsif.2017.0185.
Dabagh M, Jalali P, Butler PJ, Randles A, Tarbell JM. Mechanotransmission in endothelial cells subjected to oscillatory and multi-directional shear flow. Journal of the Royal Society, Interface. 2017 May;14(130):20170185.
Journal cover image

Published In

Journal of the Royal Society, Interface

DOI

EISSN

1742-5662

ISSN

1742-5689

Publication Date

May 2017

Volume

14

Issue

130

Start / End Page

20170185

Related Subject Headings

  • Stress, Mechanical
  • Shear Strength
  • Models, Biological
  • General Science & Technology
  • Endothelial Cells
  • Computer Simulation
  • Cell Communication
  • Cell Adhesion
  • Biomechanical Phenomena
  • Adherens Junctions