Bicarbonate alters cellular responses in respiration assays.


Journal Article

Metabolic assay buffers often omit bicarbonate, which is susceptible to alkalinisation in an open environment. Here, we assessed the effect of including bicarbonate in respirometry experiments. By supplementing HEPES-buffered media with low concentrations of bicarbonate, we found increased respiration in adipocytes and hepatocytes, but not myotubes. This was observed across multiple respirometry platforms and was independent of effects on enhanced insulin sensitivity, pH drift, or mitochondrial function. Permeabilised cell experiments suggest that bicarbonate increases substrate availability, likely by acting as a cofactor for carboxylase enzymes. This emphasises the importance of buffer choice in experimental biology.

Full Text

Duke Authors

Cited Authors

  • Krycer, JR; Fisher-Wellman, KH; Fazakerley, DJ; Muoio, DM; James, DE

Published Date

  • August 5, 2017

Published In

Volume / Issue

  • 489 / 4

Start / End Page

  • 399 - 403

PubMed ID

  • 28559140

Pubmed Central ID

  • 28559140

Electronic International Standard Serial Number (EISSN)

  • 1090-2104

Digital Object Identifier (DOI)

  • 10.1016/j.bbrc.2017.05.151


  • eng

Conference Location

  • United States